SSEA-4 Antibody (MC-813-70) [Fluorescein]

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1435F, filled histogram) or isotype control antibody (Catalog # IC007F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: MC-813-70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

SSEA-4 Antibody (MC-813-70) [Fluorescein] Summary

• Immunogen: 2120Ep human embryonal carcinoma cell line
• Specificity: Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-4 Antibody (MC-813-70) [Fluorescein]

• SSEA4
• SSEA-4
• SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

1. Shevinsky, L.H. et al. (1982) Cell 30:697.
2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-4 Antibody (FAB1435F)(9)

Publications using FAB1435F

• Breanna S. Borys, Tiffany Dang, Tania So, Leili Rohani, Tamas Revay, Tylor Walsh et al. Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors Stem Cell Research & Therapy 2021-12-01 [PMID: 33436078]
• Breanna S. Borys, Tania So, James Colter, Tiffany Dang, Erin L. Roberts, Tamas Revay et al. Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors Stem Cells Translational Medicine 2020-09-01 [PMID: 32445290]
• K Matsuura, M Wada, K Sakaguchi, Y Matsuhashi, T Shimizu Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates Regen Ther, 2019-04-25;12(0):6-13. 2019-04-25 [PMID: 31890761] (Flow Cytometry, Human)
• N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line Stem Cell Res, 2019-08-22;40(0):101547. 2019-08-22 [PMID: 31479876] (Flow Cytometry, Human)
• Hidenori Akutsu, Masakazu Machida, Seiichi Kanzaki, Tohru Sugawara, Takashi Ohkura, Naoko Nakamura et al. Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells Regenerative Therapy 2015-06-01 [PMID: 31245438]
• B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50 Stem Cell Res, 2018-09-06;32(0):78-82. 2018-09-06 [PMID: 30219716] (Flow Cytometry, Human)
• V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene Stem Cell Res, 2017-12-18;27(0):25-29. 2017-12-18 [PMID: 29304398] (Flow Cytometry, Human)
• Qiang Shi, Vida Hodara, Calvin R. Simerly, Gerald P. Schatten, John L. VandeBerg Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons Stem Cells and Development 2013-02-15 [PMID: 22931470]
• Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells, 2012-07-01;30(7):1455-64. 2012-07-01 [PMID: 22495904] (Flow Cytometry, Human)