SSEA-4 Antibody (MC-813-70) [Allophycocyanin]

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: MC-813-70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

SSEA-4 Antibody (MC-813-70) [Allophycocyanin] Summary

• Immunogen: 2120Ep human embryonal carcinoma cell line
• Specificity: Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-4 Antibody (MC-813-70) [Allophycocyanin]

• SSEA4
• SSEA-4
• SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

1. Shevinsky, L.H. et al. (1982) Cell 30:697.
2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-4 Antibody (FAB1435A)(26)

Publications using FAB1435A

• Broca-Brisson, L;Harati, R;Disdier, C;Mozner, O;Gaston-Breton, R;Maïza, A;Costa, N;Guyot, AC;Sarkadi, B;Apati, A;Skelton, MR;Madrange, L;Yates, F;Armengaud, J;Hamoudi, R;Mabondzo, A; Deciphering neuronal deficit and protein profile changes in human brain organoids from patients with creatine transporter deficiency eLife 2023-10-13 [PMID: 37830910] (Flow Cytometry, Human)
• M Moya-Jódar, A Ullate-Ago, P Barlabé, JR Rodríguez-, G Abizanda, C Barreda, X Carvajal-V, A Vilas-Zorn, JP Romero, L Garate, X Agirre, G Coppiello, F Prósper, XL Aranguren Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures Stem Cell Reports, 2022-12-22;0(0):. 2022-12-22 [PMID: 36563688] (Flow Cytometry, Human)
• Dóra Reé, Ábel Fóthi, Nóra Varga, Orsolya Kolacsek, Tamás I. Orbán, Ágota Apáti Partial Disturbance of Microprocessor Function in Human Stem Cells Carrying a Heterozygous Mutation in the DGCR8 Gene Genes (Basel) 10/23/2022 [PMID: 36360162]
• S Balasubram, M Andreani, JG Andrade, T Saha, D Sundaravin, J Garzón, W Zhang, O Popp, SI Hiraga, A Rahjouei, DB Rosen, P Mertins, BT Chait, AD Donaldson, M Di Virgili Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region Elife, 2022-04-13;11(0):. 2022-04-13 [PMID: 35416772] (IF, Human)
• D Gill, A Parry, F Santos, H Okkenhaug, CD Todd, I Hernando-H, TM Stubbs, I Milagre, W Reik Multi-omic rejuvenation of human cells by maturation phase transient reprogramming Elife, 2022-04-08;11(0):. 2022-04-08 [PMID: 35390271] (ICC/IF, Human)
• Bruno Ghirotto, Danyllo F. Oliveira, Marcella Cipelli, Paulo J. Basso, Jean de Lima, Cristiane N. S. Breda et al. MS‐Driven Metabolic Alterations Are Recapitulated in iPSC‐Derived Astrocytes Annals of Neurology 2022-05-01 [PMID: 35226368]
• Leyre López-Muneta, Javier Linares, Oscar Casis, Laura Martínez-Ibáñez, Arantxa González Miqueo, Jaione Bezunartea et al. Generation of NKX2.5GFP Reporter Human iPSCs and Differentiation Into Functional Cardiac Fibroblasts Frontiers in Cell and Developmental Biology 2022-01-21 [PMID: 35127713]
• EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research Stem Cell Research, 2021-06-24;54(0):102446. 2021-06-24 [PMID: 34216981] (ICC, Human)
• EC Pandolfi, TJ Hunt, S Goldsmith, K Hurlbut, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (UCLAi005-A, UCLAi005-B and UCLAi005-C) for reproductive science research Stem Cell Research, 2021-06-08;54(0):102409. 2021-06-08 [PMID: 34130154] (IHC, Human)
• Justin Thomas, Ludovic Zimmerlin, Jeffrey S. Huo, Michael Considine, Leslie Cope, Elias T. Zambidis Running the full human developmental clock in interspecies chimeras using alternative human stem cells with expanded embryonic potential npj Regenerative Medicine 2021-05-17 [PMID: 34001907]
• Xianzhong Lau, Pearly Jean Ai Yong, Charles Kang Liang Lou, Prabhakaran Munusamy, Mahesh Sangrithi Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry STAR Protocols 2021-03-01 [PMID: 33532739]
• P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule Cell Rep, 2019-12-10;29(11):3488-3505.e9. 2019-12-10 [PMID: 31825831] (Flow Cytometry, Human)
• EC Pandolfi, EJ Rojas, E Sosa, JJ Gell, TJ Hunt, S Goldsmith, Y Fan, SJ Silber, AT Clark Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research Stem Cell Res, 2019-09-16;40(0):101576. 2019-09-16 [PMID: 31622877] (Flow Cytometry, Human)
• N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant-Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) Stem Cell Res, 2018-09-21;32(0):145-149. 2018-09-21 [PMID: 30296667] (Flow Cytometry, Human)
• M Dejosez, TP Zwaka Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts Stem Cell Res, 2017-12-02;26(0):44-46. 2017-12-02 [PMID: 29227831] (Flow Cytometry, Human)
• A Terray, V Fort, A Slembrouck, C Nanteau, JA Sahel, S Reichman, I Audo, O Goureau Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation Stem Cell Res, 2017-10-07;25(0):26-29. 2017-10-07 [PMID: 29040912] (Flow Cytometry, Human)
• N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi001-A) and non-demented control (ASUi002-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant Stem Cell Res, 2017-06-08;24(0):160-163. 2017-06-08 [PMID: 29034886] (Flow Cytometry, Human)
• A Terray, A Slembrouck, C Nanteau, C Chondroyer, C Zeitz, JA Sahel, I Audo, S Reichman, O Goureau Generation of an induced pluripotent stem cell (iPSC) line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene Stem Cell Res, 2017-08-05;24(0):1-4. 2017-08-05 [PMID: 29034877] (Flow Cytometry, Human)
• Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells. Stem Cells, 2015-05-01;33(5):1554-65. 2015-05-01 [PMID: 25537923] (Flow Cytometry, Human)
• Paul W. Burridge, Elena Matsa, Praveen Shukla, Ziliang C. Lin, Jared M. Churko, Antje D. Ebert et al. Chemically Defined and Small Molecule-Based Generation of Human Cardiomyocytes Nature Methods 2014-08-01 [PMID: 24930130]
• Zhu H, Hu S, Baker J JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells. Stem Cells, 2014-08-01;32(8):2098-110. 2014-08-01 [PMID: 24740926] (Flow Cytometry, Human)
• Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies. PLoS ONE, 2013-01-29;8(1):e55319. 2013-01-29 [PMID: 23383153] (Flow Cytometry, Human)
• Tea Soon Park, Ludovic Zimmerlin, Elias T. Zambidis Efficient and simultaneous generation of hematopoietic and vascular progenitors from human induced pluripotent stem cells Cytometry Part A 2013-01-01 [PMID: 22736485]
• Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice? Stem Cells, 2010-01-01;28(1):174-6. 2010-01-01 [PMID: 19924769] (Flow Cytometry, Human)
• Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells. Fertil. Steril., 2008-11-19;93(3):976-85. 2008-11-19 [PMID: 19022437] (Flow Cytometry, Human)
• Neganova I, Zhang X, Atkinson S, Lako M Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells. Oncogene, 2008-09-22;28(1):20-30. 2008-09-22 [PMID: 18806832] (Flow Cytometry, Human)