Western blot shows recombinant HCoV-229E-S RBD. PVDF membrane was probed with 1 µg/mL of Mouse Anti-HCoV-HKU1 Spike RBD Monoclonal Antibody (Catalog # MAB10938) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Spike RBD was detected in immersion fixed CHO Chinese hamster ovary cell line transfected with HCoV-229E (positive staining) and CHO Chinese hamster ovary cell line (non-transfected, negative staining) using Mouse ...read more
Simple Western lane view recombinant HCoV-229E-S RBD, loaded at 0.2 mg/mL. A specific band was detected for Spike RBD at approximately 47 kDa (as indicated) using 20 µg/mL of Mouse Anti-HCoV-229E Human Coronavirus ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Spike RBD Antibody (1045017) [Unconjugated]
Spike RBD
Background
HCoV-229E belongs to a family of viruses known as coronaviruses that are
commonly comprised of a large plus-strand RNA genome and four structural
proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and
Nucleocapsid protein (N). HCoV-229E is a
member of the alpha-coronavirus family and was discovered in 1966 (1, 2). Other well-known human coronaviruses include three
viruses that cause relatively mild respiratory disease: HCoV-NL63, HCoV-HKU1
and HCov-OC43, plus three viruses that caused the Severe Acute Respiratory Syndrome
(SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global
pandemic Covid-19 (SARS-CoV2). HCov-229E Spike Protein (S Protein) is a glycoprotein
that mediates membrane fusion and viral entry. As
with most coronaviruses, proteolytic cleavage of the S protein generates two
distinct peptides, S1 and S2 subunits. The S1 subunit is focused on attachment
of the protein to the host receptor while the S2 subunit is involved with cell
fusion. Although HCoV-229E S
protein shares high homology (56%) with HCoV-NL63, it does not employ Angiotensin-Converting
Enzyme 2 (ACE2) as the receptor like HCoV-NL63. Instead, HCoV-229E engages CD13
(aminopeptidase N) for cellular entry and replication (3). The receptor binding
domain (RBD) of HCoV-229E is solely responsible for receptor binding through
three extended receptor binding loops (4).
Hamre, D. and J.J. Procknow (1966) Proc. Soc. Exp. Biol. Med. 121:190.
Van der Hoek, L. et al. (2004) Nat. Med. 10:368.
Yeager, C.L. et al. (1992) Nature 357:420.
Wong, A.H.M. et al. (2017) Nat. Commun. 8:1735.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Spike RBD Antibody (MAB10938)(1)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: Immunocytochemistry.
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