HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike RBD protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB105401) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike RBD protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB10540) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for SARS-CoV-2 Spike RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant HCoV-229E-S RBD. PVDF membrane was probed with 1 µg/mL of Mouse Anti-HCoV-HKU1 Spike RBD Monoclonal Antibody (Catalog # MAB10938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant HCoV-NL63 S1 RBD, recombinant SARS-CoV-2 Spike S1 RBD, recombinant HCoV-HKU1 S1 RBD, recombinant HCoV-229E S1 RBD, and recombinant HCoV-OC43 S. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HCoV-NL63 Spike RBD Monoclonal Antibody (Catalog # MAB11037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

10605-CV) to immobilized Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) coated at 1 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding." class="big_lightbox"> 10605-CV) to immobilized Recombinant Human ACE-2 Fc Chimera Protein (10544-ZN) coated at 1 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant SARS-Cov-2 Spike RBD His-tagged protein (10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105802) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows recombinant SARS-CoV-2 Spike S1 RBD. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB10827) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Spike RBD at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB10827) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> 10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB10827) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Spike RBD was detected in immersion fixed Transfected & absent in Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB11292) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB003). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

933-ZN) to Recombinant SARS-CoV-2 B.1.525 Spike immobilized at 0.5 ug/mL (100 µL/well)." class="big_lightbox"> 933-ZN) to Recombinant SARS-CoV-2 B.1.525 Spike immobilized at 0.5 ug/mL (100 µL/well)." class="big_thumb" />

10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105808) but not by (B) Mouse IgG1 Isotype Control (MAB002). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105808) but not by (B) Mouse IgG1 Isotype Control (MAB002). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant SARS-Cov-2 Spike RBD His-tagged protein (10500-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # MAB105808) but not by (B) Mouse IgG1 Isotype Control (MAB002). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

DY990). Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (MAB105806) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (MAB105806) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

DY990). Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (MAB105807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (MAB105807) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Spike RBD was detected in immersion fixed Transfected & absent in Wild Type HEK293 Human Embryonic Kidney Cell Line using Mouse Anti-SARS-CoV-2 Spike RBD Monoclonal Antibody (Catalog # MAB11294) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

10780-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-SARS2-20A.EU2 S1 Antibody (Catalog # MAB11294) but not by (B) Mouse IgG1 Isotype Control (MAB002). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> 10780-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 25 µg/mL of Mouse Anti-SARS2-20A.EU2 S1 Antibody (Catalog # MAB11294) but not by (B) Mouse IgG1 Isotype Control (MAB002). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

933-ZN) to Recombinant SARS-CoV-2 20A.EU2 Spike S1 Subunit His-tag (10780-CV) immobilized at 0.2 ug/mL (100 µL/well)." class="big_lightbox"> 933-ZN) to Recombinant SARS-CoV-2 20A.EU2 Spike S1 Subunit His-tag (10780-CV) immobilized at 0.2 ug/mL (100 µL/well)." class="big_thumb" />

10499-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Llama Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # LMAB12503) but not by (B) Llama IgG1 Control (AB-011-C). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_lightbox"> 10499-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Llama Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # LMAB12503) but not by (B) Llama IgG1 Control (AB-011-C). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol. " alt="In a functional flow cytometry test, Recombinant SARS-Cov-2 Spike RBD Fc chimera protein (10499-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Llama Anti-SARS-Cov-2 Spike RBD Monoclonal Antibody (Catalog # LMAB12503) but not by (B) Llama IgG1 Control (AB-011-C). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_thumb" />

IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

10622-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of SARS-Cov-2 Spike RBD Llamabody VHH His-tag Monoclonal Antibody (Catalog # LMAB10869) but not by (B) Llama IgG1 Control. Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> 10622-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of SARS-Cov-2 Spike RBD Llamabody VHH His-tag Monoclonal Antibody (Catalog # LMAB10869) but not by (B) Llama IgG1 Control. Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant SARS-Cov-2 Spike 1 Fc-tagged protein (10622-CV) binds to HEK293 human embryonic kidney cell line transfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 50 µg/mL of SARS-Cov-2 Spike RBD Llamabody VHH His-tag Monoclonal Antibody (Catalog # LMAB10869) but not by (B) Llama IgG1 Control. Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

10788-CV) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 25 μg/mL of Mouse Anti-SARS2-RBD Antibody (Catalog #MAB11290) but not by (B) Mouse IgG2B Isotype Control (MAB004). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> 10788-CV) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 25 μg/mL of Mouse Anti-SARS2-RBD Antibody (Catalog #MAB11290) but not by (B) Mouse IgG2B Isotype Control (MAB004). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="In a functional flow cytometry test, Recombinant SARS-CoV2-RBD-E484K N501Y His-tagged protein (10788-CV) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human ACE-2 and eGFP. (A) Binding is completely blocked by 25 μg/mL of Mouse Anti-SARS2-RBD Antibody (Catalog #MAB11290) but not by (B) Mouse IgG2B Isotype Control (MAB004). Protein binding was detected with Mouse Anti-His APC-conjugated Monoclonal Antibody (IC050A). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

933-ZN) to Recombinant SARS-CoV-2 E484K N501Y Spike RBD His-tag (10788-CV) immobilized at 0.5 ug/mL (100 µL/well)." class="big_lightbox"> 933-ZN) to Recombinant SARS-CoV-2 E484K N501Y Spike RBD His-tag (10788-CV) immobilized at 0.5 ug/mL (100 µL/well)." class="big_thumb" />