Sodium Calcium Exchanger 1/NCX1 Antibody (6H2)

Product Discontinued

Sodium Calcium Exchanger 1/NCX1 Antibody (6H2) Summary

• Description: PLEASE NOTE: 0.02mg size is provided in liquid format.
• Immunogen: Fusion protein from the N-terminal region of rabbit renal NaCX
• Localization: Plasma Membrane
• Specificity: Specific for the ~120k and 160k Na+/Ca2+ exchanger 1 protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC8A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: NB300-127 is useful for Western blot and immunohistochemistry. Immunocytochemistry/Immunofluorescence was reported in scientific literature (PMID 22285480). NOTE: One should see a band at ~120 kD in Western Blot. Some tissues have a form that also runs at ~160 kD.
• Theoretical MW: 108.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Rabbit. Canine reactivity reported in scientific literature (PMID: 18025087). Chicken reactivity reported in scientific literature (PMID: 15528161). Mouse reactivity reported in scientific literature (PMID: 26068603). Rat reactivity reported in scientific literature (PMID: 22285480). Reactivity assumed based on sequence identity to a wide variety of mammalian species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 5mM Ammonium Bicarbonate
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 50 ul PBS to desired concetration.

Alternate Names for Sodium Calcium Exchanger 1/NCX1 Antibody (6H2)

• FLJ37694
• FLJ43417
• Na(+)/Ca(2+)-exchange protein 1
• NCX1
• Slc8a1
• Sodium Calcium Exchanger 1
• sodium/calcium exchanger 1
• solute carrier family 8 (sodium/calcium exchanger), member 1

Background

The expression and protein levels of the Na(+)/Ca(2+) exchanger (NCX-1) vary during heart development and in response to changes in thyroid hormone levels (1). The expression of the NCX-1 gene is two-fold higher in the hearts of fetal versus adult mice. In addition, NCX-1 mRNA and protein levels were upregulated in hypothyroidic hearts. In hyperthyroidic hearts this response was reversed, however.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sodium Calcium Exchanger 1/NCX1 Antibody (NB300-127)(8)

Publications using NB300-127

• Mareedu S, Pachon RE, Jayapalraj T et al. Sarcolipin haploinsufficiency prevents dystrophic cardiomyopathy in mdx mice Am J Physiol Heart Circ Physiol 2020-11-20 [PMID: 33216625]
• Kho Changwon, Lee Ahyoung, Jeong Dongtak et al. Small-molecule activation of SERCA2a SUMOylation for the treatment of heart failure. Nature Communications 2015-01-01 [PMID: 26068603] (WB, WB, Mouse)
• Rapti K, Stillitano F, Karakikes I et al. Effectiveness of gene delivery systems for pluripotent and differentiated cells Molecular Therapy — Methods & Clinical Development . 2015-01-01 [PMID: 26052535] (WB, Human)
• Okamoto Y, Takano M, Ohba T, Ono K. Arrhythmogenic coupling between the Na(+)-Ca(2+) exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes. J Mol Cell Cardiol;52(5):988-97. 2012-05-01 [PMID: 22285480] (ICC/IF, Rat)
• agin, O et al. Inverse correlation between the extent of N-glycan branching intercellular adhesion in epithelia: contribution of the Na,K-ATPase Beta 1 subunit. J Biol Chem. 2007-11-01 [PMID: 18025087] (WB, Canine)
• Centeno VA, Diaz de Barboza GE, Marchionatti AM et al. Dietary calcium deficiency increases Ca2+ uptake and Ca2+ extrusion mechanisms in chick enterocytes. Comp Biochem Physiol A Mol Integr Physiol 2004-10-01 [PMID: 15528161] (Chicken)
• Sears, CE et al. Cardiac neuronal nitric oxide synthase isoform regulates myocardial contraction calcium hling. Circ Res 92:e52-9. 2003-01-01 [PMID: 12623875]
• Beauge, L et al. Regulation of phosphatidylinositol-4, 5-biphosphate bound to the bovine cardiac Na+/Ca2+ exchanger. Ann N Y Acad Sci976:288-99. 2002-01-01 [PMID: 12502572]

FAQs for Sodium Calcium Exchanger 1/NCX1 Antibody (NB300-127). (Showing 1 - 2 of 2 FAQs).

1. Has NB300-127 been validated on mouse? If yes, do you have any WB images for Mouse tested by NB300-127?
   • NB300-127 has not been tested in mouse, but it has been tested in rabbit. Reactivity assumed based on sequence identity to a wide variety of mammalian species. Since rat (isoform 1) and mouse NCX1 have 100% identical sequences, there is a high chance you would be able to use it for mouse samples too.
2. Regarding the WB image on datasheet of NCX1 antibody (6H2) (NB300-127) , can you confirm if lane 2 is a rabbit heart sample? In addition, can you provide the dilution fold and loading mount used to obtain this image? Lastly, would you happen to know if the sample was tested under reducing and denaturing conditions?
   • Yes, lane 2 in the blot image of NCX1 antibody (6H2) (NB300-127) is from rabbit heart. This image was obtained by testing #NB300-127 at a dilution of 1:1000 in reducing and denaturing conditions.

Bioinformatics

• Gene Symbol: SLC8A1
• Entrez:
  • 6546(Human)
  • 20541(Mouse)
  • 29715(Rat)
• Uniprot:
  • P32418(Human)