SMPDL3B Overexpression Lysate

Images

 
Western Blot: SMPDL3B Overexpression Lysate (Adult Normal) [NBL1-16252] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMPDL3B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SMPDL3B Overexpression Lysate Summary

Description
Transient overexpression lysate of sphingomyelin phosphodiesterase, acid-like 3B (SMPDL3B), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC204846 and based on accession number NM_001009568. The protein contains a C-MYC/DDK Tag.
Gene
SMPDL3B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SMPDL3B Overexpression Lysate

  • acid sphingomyelinase-like phosphodiesterase 3b
  • ASML3BASM-like phosphodiesterase 3b
  • EC 3.1.4.-
  • sphingomyelin phosphodiesterase, acid-like 3B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-45889
Species: Hu
Applications: WB, IHC, IHC-Fr
NBP2-67309
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-44743
Species: Hu, Pm, Pm, Bv(-), Ca(-), Po(-), Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IF
NB500-330
Species: Hu
Applications: Flow, IHC, IHC-Fr, IHC-P, IP, B/N, CyTOF-ready

Publications for SMPDL3B Lysate (NBL1-16252) (0)

There are no publications for SMPDL3B Lysate (NBL1-16252).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SMPDL3B Lysate (NBL1-16252) (0)

There are no reviews for SMPDL3B Lysate (NBL1-16252). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SMPDL3B Lysate (NBL1-16252) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-16252

Additional SMPDL3B Products

Bioinformatics Tool for SMPDL3B Lysate (NBL1-16252)

Discover related pathways, diseases and genes to SMPDL3B Lysate (NBL1-16252). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SMPDL3B Lysate (NBL1-16252)

Discover more about diseases related to SMPDL3B Lysate (NBL1-16252).
 

Pathways for SMPDL3B Lysate (NBL1-16252)

View related products by pathway.

Blogs on SMPDL3B

There are no specific blogs for SMPDL3B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SMPDL3B Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SMPDL3B