SMPDL3B Overexpression Lysate


Western Blot: SMPDL3B Overexpression Lysate (Adult Normal) [NBL1-16252] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMPDL3B.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

SMPDL3B Overexpression Lysate Summary


SMPDL3B Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC204846

Accession#: NM_001009568

Protein Tag: C-MYC/DDK


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SMPDL3B Overexpression Lysate

  • acid sphingomyelinase-like phosphodiesterase 3b
  • ASML3B
  • ASML3BASM-like phosphodiesterase 3b
  • EC 3.1.4.-
  • sphingomyelin phosphodiesterase, acid-like 3B


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Gene Symbol SMPDL3B