Shigella Antibody

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.5 mg/ml

Order Details

Shigella Antibody Summary

Immunogen
Mixture of S. boydii (ATCC #8700), S. flexneri (ATCC #29903) and S. dysenteriae (ATCC #13313).
Specificity
Shigella
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ICC/IF: Works with stool samples. Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% Acetone for 5 minutes at room temperature. Air dry. Stain.
Reviewed Applications
Read 1 Review rated 3
using
NB110-11566 in the following applications:

Publications
Read Publication using
NB110-11566 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Reacts with Shigella species including S. sonnei.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
4.5 mg/ml
Purity
Protein A purified

Alternate Names for Shigella Antibody

  • Shigella Boydii; Shigella Dysenteriae; Shigella Flexneri; Shigella Sonnei

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Shigella Antibody (NB110-11566)(1)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Review for Shigella Antibody (NB110-11566) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB110-11566:
Filter by Applications
IHC-P
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Other 03/11/2009
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPig tissue section-intestines
SpeciesOther
Lot6A02606

Blocking

Blocking DetailsBlocking Buffer: Mixture of bovine serum albumin (BSA 0.05%) and goat normal serum, Blocking Time/Temp: 30 min at RT

Primary Anitbody

Dilution RatioDilution Ratio: 1/400 - 1/800, Dilution Buffer: Phosphate-buffered saline (PBS 0.01M pH7.2) w/ 0.05% BSA, Time/Temp:1.5 hrs @ RT

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit IgG
Secondary Manufacturer Cat#ABC Kit from Vector Laboratories

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Shigella Antibody (NB110-11566) (0)

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Anonymous
03/11/2009
Application: IHC-P
Species: Other