Serotonin Antibody (YC5/45) - Azide and BSA Free

Immunohistochemistry: Serotonin Antibody (YC5/45) - Azide and BSA Free [NBP2-80946] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Serotonin antibody (clone YC5/45). The signal was developed using HRP-DAB method and the nuclei were counterstained using hematoxylin. This antibody generated a specific strong stain

• Reactivity: All
• Applications: ICC/IF, IHC, IHC
• Clone: YC5/45
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Serotonin Antibody (YC5/45) - Azide and BSA Free Summary

• Immunogen: Serotonin conjugated to Bovine Serum Albumin.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry Whole-Mount reported in scientific literature (PMID 7781904)
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500

Reactivity Notes

This antibody detects serotonin in multiple species including human, mouse, rat, and other vertebrate and invertebrate animals.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Serotonin Antibody (YC5/45) - Azide and BSA Free

• 5-HT
• 5-hydroxytryptamne

Background

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20um) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors. Useful for the diagnosis of carcinoid tumors and the investigation of the effects of drugs on the neurological system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.