Serotonin ELISA Kit (Colorimetric)

ELISA: Serotonin ELISA Kit [KA1894] - Standard curve

ELISA: Serotonin ELISA Kit (Colorimetric) [KA1894] - Image from verifed customer review.

• Applications: ELISA, ELISA(Comp), NULL
• Suitable Sample Type: Platelets, Serum, Urine
• Standard Curve Range: 10.2 to 2,500 ng/mL
• Sensitivity: 6.2 ng/mL

Serotonin ELISA Kit (Colorimetric) Summary

• Description: Serotonin ELISA Kit is an enzyme immunoassay for the quantitative determination of Serotonin in serum, urine and platelets.
• Standard Curve Range: 10.2 to 2,500 ng/mL
• Sensitivity: 6.2 ng/mL
• Assay Type: Quantification
• Inter-Assay: Urine: 7.65%, Serum: 6.0%
• Intra-Assay: Urine: 6.5%, Serum: 4.65%
• Spike Recovery: Urine: 94%, Serum: 97%
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Competitive ELISA
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Serotonin ELISA Kit (Colorimetric)

• 5-HT
• 5-hydroxytryptamne

Background

In the first step, Serotonin is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Serotonin ELISA Kit (KA1894)(13)

Publications using KA1894

• Wong AC, Devason AS, Umana IC et al. Serotonin reduction in post-acute sequelae of viral infection Cell 2023-10-26 [PMID: 37848036]
• Alamri HS Talbinah (Barley Porridge): neurotransmitters modulatory effect and antidepressant-like action in experimentally depressed rats European review for medical and pharmacological sciences 2022-01-01 [PMID: 35048996] (ELISA)
• Vincenzo N, Silvana G, Francesco P et al. The Effect of a Probiotic Complex on the Gut-Brain Axis: A Translational Study. Neuropsychobiology. 2021-08-30 [PMID: 34515196]
• Melissa V, An H, Michel M et al. Serotonin Levels in the Serum of Persons with Onchocerciasis-Associated Epilepsy: A Case-Control Study. Pathogens. 2021-06-08 [PMID: 34201076]
• A Dickhout, BME Tullemans, JWM Heemskerk, VLJL Thijssen, MJE Kuijpers, RR Koenen Galectin-1 and platelet factor 4 (CXCL4) induce complementary platelet responses in vitro PLoS ONE, 2021-01-07;16(1):e0244736. 2021-01-07 [PMID: 33411760]
• Aleksovski B, Neceva V, Vujovi? V et al. SSRI-reduced platelet reactivity in non-responding patients with life-long Recurrent Depressive Disorder: Detection and involved mechanisms. Thromb Res 2018-03-10 [PMID: 29549779] (ELISA, Human)
• Varshney MK, Inzunza J, Lupu D et al. Role of estrogen receptor beta in neural differentiation of mouse embryonic stem cells. Proc Natl Acad Sci U S A 2017-11-13 [PMID: 29133394]
• Chalermkitpanit P, Thonnagith A, Engsusophon P et al. Noradrenaline, Serotonin, GABA, and Glycine in Cerebrospinal Fluid during Labor Pain: A Cross-Sectional Prospective Study. Pain Res Manag 2017-06-18 [PMID: 28701860]
• Cremon C, Stanghellini V, Barbaro MR et al. Randomised clinical trial: the analgesic properties of dietary supplementation with palmitoylethanolamide and polydatin in irritable bowel syndrome. Aliment Pharmacol Ther 2017-02-06 [PMID: 28164346]
• Dong W, Liu Z, Qiu L et al. The modulation role of serotonin in Pacific oyster Crassostrea gigas in response to air exposure. Fish Shellfish Immunol 2017-03-01 [PMID: 28159695]
• Al-Rawaf, HA, Alghadir, AH, Gabr SA Circulating microRNAs and Molecular Oxidative Stress in Older Adults with Neuroprogression Disorders Disease markers 2021-10-22 [PMID: 34721735]
• Cox RT The Impact of Physical Education on Students' Performance Outcomes in Mathematics Thesis 2022-01-01 (ELISA)

Review for Serotonin ELISA Kit (KA1894) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 01/23/2019

Summary

• Sample Tested: Animal Component Free Media,Positive Controls (Included),RPMI + 10% FBS
• Lot: 180442

FAQs for Serotonin ELISA Kit (KA1894). (Showing 1 - 1 of 1 FAQs).

1. Can this kit be used with rat serum/plasma?
   • Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.