SARS-CoV-2 Spike S1 Protein Antibody (1038459) [Unconjugated]

Spike S1 Subunit was detected in immersion fixed CHO Chinese hamster ovary cell line transfected with MERS Spike S1 (positive staining) and CHO Chinese hamster ovary cell line (non-transfected, negative staining) using Mouse Anti-MERS-CoV Spike S1 Subunit Monoclonal Antibody (Catalog # MAB10707) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: V
• Applications: ICC/IF
• Clone: 1038459
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SARS-CoV-2 Spike S1 Protein Antibody (1038459) [Unconjugated] Summary

• Immunogen: Spodoptera frugiperda insect ovarian cell line Sf21-derived MERS-CoV-2 Spike S1
  Met1-Pro747
  Accession # K9N5Q8.1
• Specificity: Detects MERS-CoV-2 Spike S1 in ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SARS-CoV-2 Spike S1 Protein Antibody (1038459) [Unconjugated]

• SARS-CoV-2
• Spike S1 Subunit

Background

MERS-CoV (also known as HCoV-EMC), which causes the Middle East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1,2). Other well-known human coronaviruses include several viruses that cause relatively mild respiratory disease, plus two viruses that caused the Severe Acute Respiratory Syndrome (SARS-CoV) and the global pandemic Covid-19 (SARS-CoV2). MERS-CoV Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry, and it consists of two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3). Based on amino acid (aa) sequence homology, the MERS-CoV S1 subunit shares 23% and 22% identity with SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence homology is consistent with the finding that MERS-CoV and SARS-CoV bind different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage ACE2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4 (DPP4; also known as CD26) as its functional receptor (4). Based on structural biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is located in the C-terminal region of S1 subunit and consists of a core subdomain and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region, was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).

1. Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
2. Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
3. Li, Y. et al. (2019) Engineering. 5:940.
4. Raj, V.S. et al. (2013) Nature 495:251.
5. Lu, G. et al. (2013) Nature 500:227.
6. Wang, N. et al. (2013) Cell. Res. 23:986.
7. Corti, D. et al. (2016) J. Infect. Public Health 9:231.
8. Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
9. Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • K9N5Q8.1()