SARS-CoV-2 Spike S1 Protein Products

HAF018). A specific band was detected for Spike S1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">

10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105403) or (B) Mouse IgG1 Isotype Control Antibody (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank">

10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105403) or (B) Mouse IgG1 Isotype Control Antibody (MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Prior to staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to SARS-CoV-2 infected cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in bronchial epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

HAF008). A specific band was detected for Spike S1 RBD at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to immunoreactive profiles scattered throughout the tissue. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">

11056-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank">

10522-CV), then stained with (A) Mouse Anti-SARS-CoV-2 Spike S1 Monoclonal Antibody (Catalog # MAB105805) or (B) Mouse IgG2A Isotype Control Antibody (MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox" target="_blank">

933-ZN) in a functional ELISA." class="big_lightbox" target="_blank">

10569-CV) was immobilized on a Biacore Sensor Chip CM5, and binding to recombinant human ACE-2 (933-ZN) was measured at a concentration range between 0.184 nM and 93.5 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of K_D=2.625 nM. " class="big_lightbox" target="_blank">

10522-CV) binds Recombinant Human ACE-2 His-tag (933-ZN) in a functional ELISA." class="big_lightbox" target="_blank">

10522-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-92 kDa and 70-80 kDa, respectively." class="big_lightbox" target="_blank">

IC050A), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox" target="_blank">

10544-ZN) in a functional ELISA." class="big_lightbox" target="_blank">

F0050), which does not stain the cells in the absence of recombinant protein (B)." class="big_lightbox" target="_blank">