![Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 u/mL and Lane 2: 2 u/mL](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202419531663.gif "Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 u/mL and Lane 2: 2 u/mL")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | SARS-CoV-2 pp1ab polyprotein antibody was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 pp1ab polyprotein protein. The immunogen is located within 120-170 amino acids of the SARS-CoV-2 pp1ab polyprotein protein. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ORF1ab |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ORF1ab |
![ELISA: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, . A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202420324846.jpg "ELISA: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, . A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL")
![Immunohistochemistry: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (0.5 u/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202420432392.jpg "Immunohistochemistry: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (0.5 u/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
