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![Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 u/mL and Lane 2: 2 u/mL](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202419531663.gif "Western Blot: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP16 recombinant protein (10-418).Antibodies: SARS-CoV-2 (COVID-19) NSP16, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 u/mL and Lane 2: 2 u/mL")
![ELISA: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) NSP16 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP16 Antibody, . A direct ELISA was performed using SARS-CoV-2 NSP16 recombinant protein (10-418) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP16 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202420324846.jpg)
![Immunohistochemistry: SARS-CoV-2 pp1ab polyprotein Antibody [NBP3-27904] - Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) NSP16 antibody (0.5 u/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP16 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/nbp3-27904_rabbit-sars-cov-2-pp1ab-polyprotein-pab-2711202420432392.jpg)