![Western Blot: SARS-CoV-2 ORF3b Antibody [NBP3-11929] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein. Antibodies: SARS-CoV-2 ORF3b, NBP3-11929, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL and Lane 2: 0.5 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF3b-Antibody-Western-Blot-NBP3-11929-img0002.jpg "Western Blot: SARS-CoV-2 ORF3b Antibody [NBP3-11929] - Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein. Antibodies: SARS-CoV-2 ORF3b, NBP3-11929, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL and Lane 2: 0.5 ug/mL")
| Reactivity | V, VSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV-2 ORF3b protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 ORF3b protein. |
| Predicted Species | SARS-CoV (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: SARS-CoV-2 ORF3b Antibody [NBP3-11929] - ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 ORF3b Antibody, NBP3-11929. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein as coating antigen and the anti-SARS-CoV-2 ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF3b-Antibody-ELISA-NBP3-11929-img0001.jpg "ELISA: SARS-CoV-2 ORF3b Antibody [NBP3-11929] - ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 ORF3b Antibody, NBP3-11929. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein as coating antigen and the anti-SARS-CoV-2 ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")