SARS-CoV-2 Nucleocapsid Antibody (NP642) - Azide and BSA Free

Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP642) [NBP3-07072] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. Antibodies: SARS-CoV-2 Nucleocapsid Antibodies (NBP3-07072). A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.5 ng

Sandwich ELISA: SARS-CoV-2 Nucleocapsid Antibody (NP642) [NBP3-07072] - Sandwich ELISA for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng

Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (NP642) - Azide and BSA Free [NBP3-07072] - Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (1 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

• Reactivity: V
• Applications: ELISA, IHC
• Clone: NP642
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

SARS-CoV-2 Nucleocapsid Antibody (NP642) - Azide and BSA Free Summary

• Description: The antibody was expressed in HEK293F cells, and subsequently obtained from cell supernatant by protein A affinity chromatography.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant SARS-CoV-2 nucleocapsid protein (full-length).
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: N
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Sandwich ELISA
• Application Notes: Sandwich ELISAs for SARS-CoV-2 Nucleocapsid Matched Pair Antibodies. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07069, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07068, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.5 ng. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (NBP3-07072, 5ug/ml) as capture antibody, a Nucleocapsid recombinant protein as the binding protein, and the anti-SARS-CoV-2 Nucleocapsid antibody (NBP3-07070, 1ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.7 ng.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS pH 7.2
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SARS-CoV-2 Nucleocapsid Antibody (NP642) - Azide and BSA Free

• 2019-nCoV N Protein
• 2019-nCoV Nucleocapsid Protein
• COVID-19 N protein
• COVID-19 nucleocapsid protein
• Human coronavirus Nucleoprotein
• nucleocapsid phosphoprotein
• Nucleocapsid protein
• Nucleoprotein
• ORF9; structural protein
• SARS-CoV-2 Nucleocapsid protein
• SARS-CoV-2 N protein
• SARSCoV2 Nucleoprotein
• SARS-CoV-2
• Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein

Background

The SARS-CoV-2 Nucleocapsid protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The nucleocapsid protein is an RNA-binding protein that is essential for viral assembly into a ribonucleoprotein complex and also functions in viral budding (2,3). More specifically, after viral replication and RNA synthesis, the viral genomes that are encapsulated by the nucleocapsid protein will bud into the membrane and help with mature virus formation (2). The nucleocapsid protein is synthesized as 419 amino acids (aa) in length with a theoretical molecular weight of 45.6 kDa (4). Structurally, the nucleocapsid protein contains an RNA-binding domain (N1b), a dimerization domain (N2b), and a few shorter regions (N1a, N2a, and spacer B/N3) (3). It is the N1b domain that is suggested to be involved in RNA binding (3). The nucleocapsid N1b domain contains a beta-hairpin, a loop region, and a beta-sheet core, giving it a right-handed shape (3). Conversely, the N2b domain functions in dimerization which promotes assembly into a helical filament where the dimer interface is made of two beta-strands and an alpha-helix portion (3). Inhibition of nucleocapsid assembly may be a potential target for treating COVID-19 infection.

Following SARS-CoV-2 infection, B cells and T cells display an immune response against nucleocapsid protein and nucleocapsid-specific neutralizing antibodies are produced (5). Interestingly, a study of patients who have recovered from COVID-19 revealed that there were no neutralizing antibodies against nucleocapsid protein present; however, a high presence against neutralizing spike RBD antibodies were detected (5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Zhu, G., Zhu, C., Zhu, Y., & Sun, F. (2020). Minireview of progress in the structural study of SARS-CoV-2 proteins. Current Research in Microbial Sciences. https://doi.org/10.1016/j.crmicr.2020.06.003

4. Uniprot (P0DTC9)

5. Shah, V. K., Firmal, P., Alam, A., Ganguly, D., & Chattopadhyay, S. (2020). Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01949

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: N