SARS-CoV-2 nsp6 Antibody [Alexa Fluor® 532]

• Reactivity: V, V
• Applications: ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

SARS-CoV-2 nsp6 Antibody [Alexa Fluor® 532] Summary

• Immunogen: This antibody was raised against a peptide corresponding to 18 amino acids in the center of SARS-CoV-2 (COVID-19) NSP6 protein. The immunogen is located in between 230-280 amino acids of the SARS-CoV-2 (COVID-19) NSP6 protein.
• Predicted Species: SARS-CoV (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORF1ab
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SARS-CoV-2 nsp6 Antibody [Alexa Fluor® 532]

• Non-structural protein 6

Background

SARS-CoV-2 Nonstructural Protein 6 (NSP6) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP6 is 290 amino acids (aa) with a theoretical molecular weight of 33 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP6 has 88.2% sequence identity and 98.3% sequence similarity (1). Structurally, NSP6 is a transmembrane protein with six transmembrane domains (3). SARS-CoV-2 NSP6 functions in autophagosome formation and double membrane vesicle formation (1,3). A SARS-CoV-2 interactome study revealed that NSP6 has a role in vesicle trafficking. Specifically, it is shown to interact with vacuolar ATPase (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORF1ab