![Western Blot: SARS-CoV-2 nsp15 Antibody [NBP3-11932] - Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein. Antibodies: SARS-CoV-2 nsp15, NBP3-11932, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp15-Antibody-Western-Blot-NBP3-11932-img0002.jpg "Western Blot: SARS-CoV-2 nsp15 Antibody [NBP3-11932] - Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein. Antibodies: SARS-CoV-2 nsp15, NBP3-11932, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL")
| Reactivity | V, VSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 NSP15 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 NSP15 protein. |
| Predicted Species | SARS-CoV (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ORF1ab |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ORF1ab |
![ELISA: SARS-CoV-2 nsp15 Antibody [NBP3-11932] - ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein Antibodies: SARS-CoV-2 nsp15 Antibody, NBP3-11932. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein as coating antigen and the anti-SARS-CoV-2 nsp15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-nsp15-Antibody-ELISA-NBP3-11932-img0001.jpg "ELISA: SARS-CoV-2 nsp15 Antibody [NBP3-11932] - ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein Antibodies: SARS-CoV-2 nsp15 Antibody, NBP3-11932. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein as coating antigen and the anti-SARS-CoV-2 nsp15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")