SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 NSP10 Antibody [NBP3-07962] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) NSP10 Protein antibody at dilution of 1:1000.

• Reactivity: V
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SARS-CoV-2 NSP10 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant SARS-CoV-2 NSP10(4254A-S4393)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ORF1ab
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000-1:8000
  • Western Blot
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 50% glycerol.
• Preservative: 0.05% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SARS-CoV-2 NSP10 Antibody - Azide and BSA Free

• GFL
• Growth factor-like peptide
• Non-structural protein 10

Background

SARS-CoV-2 Nonstructural Protein 10 (NSP10) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP10 is 139 amino acids (aa) with a theoretical molecular weight of 14.8 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP10 has 97.1% sequence identity and 99.3% sequence similarity (1). Functionally, NSP10 interacts with NSP14 which acts as an S-adenosylmethionine (SAM)-dependent (guanine-N7) methyl transferase (N7-MTase) and stimulates it by significantly increasing exoribonuclease activity (1,3,4). Additionally, SARS-CoV-2 NSP10 is proposed to be a cofactor for NSP16, which is a 2'-O-methyltransferase, and the interaction is needed for it to become active (1-4). NSP10 can interact with both NSP14 and NSP16 simultaneously, acting as both a cofactor for each as well as a molecular connector (4). A SARS-CoV-2 interactome study also revealed that NSP10 is involved in vesicle trafficking (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORF1ab