SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] - SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] detects SARS-CoV-2 (COVID-19) nsp10 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp10 stained by SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] (NBP3-25650) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10um.

Western Blot: SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free [NBP3-25650] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] (NBP3-25650) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: V
• Applications: WB, ICC/IF
• Clone: HL1381
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length SARS-CoV-2 NSP10 recombinant protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ORF1ab
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SARS-CoV-2 NSP10 Antibody (HL1381) - Azide and BSA Free

• GFL
• Growth factor-like peptide
• Non-structural protein 10

Background

SARS-CoV-2 Nonstructural Protein 9 (NSP9) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP9 is 113 amino acids (aa) with a theoretical molecular weight of 12.4 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP9 has 97.3% sequence identity and 99.1% sequence similarity (1). Structurally NSP9 has a N-terminal beta-barrel made of seven beta-strands and a C-terminal alpha-helix (3). In SARS-CoV, NSP9 is important for viral proliferation; it is unclear if NSP9 plays the same role in SARS-CoV-2 but given the high degree of sequence similarity it is quite likely (4). SARS-CoV-2 NSP9 also plays a role in formation of nuclear transport machinery and the extracellular matrix (2). Additionally, NSP9 binds mind bomb 1 (MIB1), an E3 ubiquitin ligase that regulates antiviral innate immune signaling (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., ... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Khan, M. T., Irfan, M., Ahsan, H., Ahmed, A., Kaushik, A. C., Khan, A. S., Chinnasamy, S., Ali, A., & Wei, D. Q. (2021). Structures of SARS-CoV-2 RNA-Binding Proteins and Therapeutic Targets. Intervirology. https://doi.org/10.1159/000513686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ORF1ab