![Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein (10-400). Antibodies: SARS-CoV-2 3CL Protease (NBP3-07061), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-Western-Blot-NBP3-07061-img0001.jpg "Western Blot: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - Western Blot Validation with SARS-CoV-2 3CL Protease Protein. Loading: 30 ng per lane of SARS-CoV-2 3CL Protease recombinant protein (10-400). Antibodies: SARS-CoV-2 3CL Protease (NBP3-07061), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 ug/ml and Lane 2: 1 ug/ml")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | This antibody was raised against a peptide corresponding to 15 amino acids in the center of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. The immunogen is located within 80-130 amino acids of the SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ORF1ab |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publication using NBP3-07061 | Applications | Species |
|---|---|---|
| Planes R, Pinilla M, Santoni K et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells Molecular cell 2022-05-16 [PMID: 35594856] (WB, ICC/IF, SARS-CoV-2) | WB, ICC/IF | SARS-CoV-2 |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ORF1ab |
![ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - ELISA Validation with SARS-CoV-2 3CL Protease Protein . Antibodies: SARS-CoV-2 3CL Protease Antibody, NBP3-07061. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-3CL-Protease-Antibody-ELISA-NBP3-07061-img0002.jpg "ELISA: SARS-CoV-2 3CL Protease Antibody [NBP3-07061] - ELISA Validation with SARS-CoV-2 3CL Protease Protein . Antibodies: SARS-CoV-2 3CL Protease Antibody, NBP3-07061. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein as coating antigen and the anti-SARS-CoV-2 3CL Protease antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")