Salmonella Antibody - BSA Free

• Reactivity: Mu, Ba
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 4.5 mg/ml

Salmonella Antibody - BSA Free Summary

• Immunogen: Mixture of S. enteriditis, S. typhimurium, and S. heidelburg
• Localization: whole cell
• Specificity: This is polyvalent for Salmonella "O" & "H" antigens. Immunocaptures Salmonellae. Antiserum is not absorbed and does react with related Enterobacteriaceae.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: ELISA, Immunofluorescence. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Immunocaptures Salmonellae. Optimal dilutions and concentrations should be determined by the end user. Purity is >95% Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 23732169)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27748732).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01 M Phosphate Buffered Saline, pH 7.2
• Preservative: 0.1% Sodium Azide
• Concentration: 4.5 mg/ml
• Purity: Protein A purified

Alternate Names for Salmonella Antibody - BSA Free

• Salmonellae

Background

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Salmonella Antibody (NB600-1087)(8)

Publications using NB600-1087

• Guo XK, Wang J, van Hensbergen VP et al. Interactions between host and intestinal crypt-resided biofilms are controlled by epithelial fucosylation Cell reports 2023-07-25 [PMID: 37405914] (IHC-P, Bacteria)
• Wang DN, Ni JJ, Li JH et al. Bacterial infection promotes tumorigenesis of colorectal cancer via regulating CDC42 acetylation PLoS pathogens 2023-02-22 [PMID: 36812247] (IHC, Human)
• Ahn YH, Park S, Choi JJ et al. Secreted tryptophanyl-tRNA synthetase as a primary defence system against infection. Nat Microbiol 2016-10-17 [PMID: 27748732] (IF/IHC)
• Seong H, Kim S, Choi J et al. Synthesis of ultrasmall superparamagnetic iron oxide nanoparticles for magnetic labeling of antibody of pathogenic microbe Macromolecular Research 2023-02-01
• Habyarimana F, Sabag-Daigle A, Ahmer BM. The sdiA-regulated gene, srgE, encodes a Type 3 secreted effector. J. Bacteriol. 2014-04-11 [PMID: 24727228] (ICC/IF, Bacteria)
• Marshall JM, Flechtner AD, La Perle KM. Visualization of Extracellular Matrix Components within Sectioned Salmonella Biofilms on the Surface of Human Gallstones. PLoS ONE 2014-02-19 [PMID: 24551241] (IHC-P)
• Habyarimana F, Swearingen MC, Young GM et al. Yersinia enterocolitica inhibits Salmonella enterica serovar Typhimurium and Listeria monocytogenes cellular uptake. Infect. Immun. 2014-01-01 [PMID: 24126528] (ICC/IF, Bacteria)
  
  Details:
  Salmonella antibody used for ICC-IF at 1:500 dilution - Infected cells fixed in 4% paraformaldehyde/PBS for 15 min, two protocols explained - one each for labelling the extracellular and intracellular bacteria (see full text for details).
• Gonzalez-Escobedo G, Gunn JS. Gallbladder epithelium as a niche for chronic Salmonella carriage. Infect Immun. 2013-08-01 [PMID: 23732169] (IHC-P, Bacteria)