Salmonella Antibody

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Product Details

Summary
Reactivity BaSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
4.5 mg/ml

Order Details

Salmonella Antibody Summary

Immunogen
Mixture of S. enteriditis, S. typhimurium, and S. heidelburg
Localization
whole cell
Specificity
This is polyvalent for Salmonella "O" & "H" antigens. Immunocaptures Salmonellae. Antiserum is not absorbed and does react with related Enterobacteriaceae.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
ELISA, Immunofluorescence. May be used in place of neat antiserum in almost any appropriate antibody-based technique. Immunocaptures Salmonellae. Optimal dilutions and concentrations should be determined by the end user. Purity is >95% Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 23732169)
Publications
Read Publications using
NB600-1087 in the following applications:

Reactivity Notes

Polyvalent for Salmonella 'O' & 'H' antigens. Immunocaptures Salmonellae. Antiserum is not absorbed and does react with related Enterobacteriaceae.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
4.5 mg/ml
Purity
Protein A purified

Background

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Salmonella Antibody (NB600-1087)(5)

We have publications tested in 2 confirmed species: Mouse, Bacteria.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-P
(2)
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Filter By Species
Mouse
(1)
Bacteria
(3)
All Species

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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