![Western Blot: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - Nuclear extracts of SKNO-1 cells (15 ug) were analysed by Western blot using the antibody against AML1-ETO diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (kDa) is shown on the left, the position of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/RUNX1-and-RUNX1T1-Antibody-Western-Blot-NBP2-59160-img0004.jpg "Western Blot: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - Nuclear extracts of SKNO-1 cells (15 ug) were analysed by Western blot using the antibody against AML1-ETO diluted 1:1,000 in TBSTween containing 5% skimmed milk. The marker (kDa) is shown on the left, the position of the protein of interest is indicated on the right.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Serum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![Chromatin Immunoprecipitation: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.](http://images.novusbio.com/fullsize/RUNX1-and-RUNX1T1-Antibody-Chromatin-Immunoprecipitation-NBP2-59160-img0002.jpg "Chromatin Immunoprecipitation: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - The IP'd DNA of 6 ChIP's was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.")
![ELISA: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against AML1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32,750.](http://images.novusbio.com/fullsize/RUNX1-and-RUNX1T1-Antibody-ELISA-NBP2-59160-img0001.jpg "ELISA: RUNX1 and RUNX1T1 Antibody [NBP2-59160] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against AML1-ETO. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:32,750.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")