RNF133 Antibody (3D6)

Western Blot: RNF133 Antibody (3D6) [H00168433-M01] - Analysis of RNF133 expression in transfected 293T cell line by RNF133 monoclonal antibody (M01), clone 3D6.Lane 1: RNF133 transfected lysate(42.3 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: RNF133 Antibody (3D6) [H00168433-M01] - Detection limit for recombinant GST tagged RNF133 is 0.1 ng/ml as a capture antibody.

Product Discontinued

RNF133 Antibody (3D6) Summary

• Immunogen: RNF133 (NP_631914, 277 a.a. ~ 376 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQNNDIQPHSVVEDVHPSP
• Localization: Membrane; Single-pass membrane protein.
• Specificity: RNF133 - ring finger protein 133
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF133
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF133 Antibody (3D6)

• E3 ubiquitin-protein ligase RNF133
• EC 6.3.2.-
• ring finger protein 133MGC27072

Background

The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene has no intron.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF133
• Entrez:
  • 168433(Human)
• Uniprot:
  • NP_631914(Human)