RFP Antibody (HL2028) - Azide and BSA Free

Western Blot: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted antibodies as follows: Upper image: RFP antibody [HL2028], NBP3-25634 (1:5000) Middle image: mCherry-tagged protein antibody, NBP2-43720 Lower image: FP-tagged protein antibody, The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: RFP Antibody (HL2028) - Azide and BSA Free [NBP3-25634] - RFP-tag antibody detects RFP tagged protein by immunofluorescent analysis. Sample: RFP tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RFP tagged protein stained by RFP-tag antibody (NBP3-25634) diluted at 500. Red: RFP tagged protein is expressed in the transfected cells. Blue: Fluoroshield with DAPI .

• Reactivity: All-NA
• Applications: WB, ICC/IF
• Clone: HL2028
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RFP Antibody (HL2028) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RFP Antibody (HL2028) - Azide and BSA Free (NBP3-25634) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Full length RFP recombinant protein
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RFP Antibody (HL2028) - Azide and BSA Free

• DSRED
• GFP-like chromoprotein
• Red Fluorescent Protein
• RFP

Background

Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.