Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.5-5.0 μg/mL.

1 μg/lane of Recombinant Mouse Skint2/B7S3 Fc Chimera (Catalog # 10279-B7) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 48-64 kDa and 95-130 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.5-5.0 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Skint2/B7S3 protein
  

  Mouse Skint2/B7S3 (Ser22-Lys239) Accession # NP_001272892.1	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001272892.1
• N-terminal Sequence: Ser22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 48-64 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Skint2/B7S3 Fc Chimera Protein, CF

• B7S3
• Skint2

Background

B7S3 (B7 superfamily member 3) is a member of the B7 superfamily which play a major role in maintaining the appropriate threshold of T cell activation. B7S3 has two isoforms expressed in both lymphoid and non-lymphoid tissues (1). The mature extracellular domain (ECD) of mouse B7S3 contains an IgV-like domain, and a half IgC-like domain (1). Within the ECD, mouse B7S3 shares 89% amino acid sequence identity with rat B7S3. A putative receptor for B7S3 is constitutively expressed on APCs and induced on T cells upon activation. Mouse B7S3 mFc chimera inhibited CD4 and CD8 T cell proliferation and IL-2 production, as well as effector cytokine production. Interestingly, the B7S3 genes in mouse and human have distinct structural features (1).

1. Yang, Y. et al. (2007) J. Immunol. 178:3661.

Bioinformatics

• Uniprot:
  • NP_001272892.1()