Recombinant Mouse IFNAB Protein, CF

Recombinant Mouse IFNAB (Catalog # 10151-IF) suppressesviral activity on L-929 mouse fibroblast cells infected withencephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 pg/mL.

2 μg/lane of Recombinant Mouse IFNAB(Catalog # 10151-IF) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 17-25 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFNAB Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 0.6-6 pg/mL
• Source: Human embryonic kidney cell, HEK293-derived mouse IFNAB protein
  Cys24-Glu190
• Accession #: AAA58254.1
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17-25 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFNAB Protein, CF

• IFNAB

Background

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN alpha subtypes and the various IFN alpha share about 80% sequence homology among them (4, 5). The mouse IFNAB consists of 190 amino acids (aa) including a 23 aa signal peptide and a 167 aa IFNAB main chain. It was found that IFNAB plays an important role in the regulation of DNA synthesis in colong-stimulating factor (CSF)-1-stimulated macrophage, as well as mediates the inhibitory efffects of LPS and TNF alpha (6). IFN regulatory factor 2 (IRF-2) controls IFNAB signaling and the absence of its negative regulation selectively affects development of the CD8 alpha ^- myeloid DC population (7).

1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
6. Hamilton JA, et al. (1996) J Immunol. 156(7):2553.
7. Kenya H, et al. (2004) Proc Natl Acad Sci U S A. 101(8):2416.

Bioinformatics

• Uniprot:
  • AAA58254.1()