Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF

Recombinant Human Neuroligin 4Y/NLGN4Y (Catalog # 9119-NL) Enhances Neurite Outgrowth in E13 Chick Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Untreated E13 DRG neurons show minimal neurite outgrowth. B) Recombinant Human Neuroligin 4Y/NLGN4Y, immobilized at 10 µg/mL, enhances DRG neuron neurite outgrowth.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Neuroligin 4Y/NLGN4Y protein
  Gln42-Val677, with a C-terminal 6-His tag
• Accession #: Q8NFZ3
• N-terminal Sequence: No results obtained. Gln42 inferred from enzymatic pyroglutamate treatment revealing Ala43
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90-105 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neuroligin 4Y/NLGN4Y Protein, CF

• neuroligin 4, Y-linked
• Neuroligin 4Y
• Neuroligin Y
• neuroligin-4, Y-linked
• NLGN4Y
• Y linked

Background

Neuroligin 4 (NLGN4, NL4 or NL4X) is a type I transmembrane molecule in the type-B carboxyesterase/lipase family of proteins. Neuroligins are postsynaptically expressed on neurons and initiate excitatory presynapse maturation through binding to select isoforms of b-neurexin (1). Human NLGN4 maps to the X-chromosome and shares 98% aa identity with the Y-chromosome-encoded NLGN4Y (2). Mature human NLGN4Y consists of a 633 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 119 aa cytoplasmic tail. It is expressed in the frontal cortex, temporal cortex, occipital cortex, hippocampus, cerebellum, and spinal cord (3, 4). The ECD possesses a nonfunctional esterase homology domain through which neuroligins, except for NLGN2, interact with neurexins (5). Alternative splicing of NLGN4Y generates additional isoforms with variable N-terminal deletions and/or insertions and truncations within the ECD. NLGNs 3, 4, and 4Y bind to the intracellular adaptor protein Syntrophin-g2 (6). Mutations of NLGN4 but not NLGN4Y are associated with rare cases of autism and Asperger or Tourette syndromes (4, 7-12).

1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
2. Bolliger, M.F. et al. (2001) Biochem. J. 356:581.
3. Johansson, M.M. et al. (2016) Biol. Sex Differ. 7:5.
4. Jamain, S. et al. (2003) Nat. Genet. 34:27.
5. Chen, X. et al. (2008) Nat. Struct. Mol. Biol. 15:50.
6. Yamakawa H. et al. (2007) Biochem. Biophys. Res. Commun. 355:41.
7. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
8. Lawson-Yuen, A. et al. (2008) Eur. J. Hum. Genet. 16:614.
9. Jamain, S. et al. (2008) Proc. Natl. Acad. Sci. USA 105:1710.
10. Yan, J. et al. (2008) Psychiatr. Genet. 18:204.
11. Serajee, F.J. and A.H. Mahbubul Huq (2009) J. Child Neurol. 24:1258.
12. Ylisaukko-oja, T. et al. (2005) Eur. J. Hum. Genetics 13:1285.

Publications for Neuroligin 4Y/NLGN4Y (9119-NL)(1)

Publication using 9119-NL

• HS Venkatesh, LT Tam, PJ Woo, J Lennon, S Nagaraja, SM Gillespie, J Ni, DY Duveau, PJ Morris, JJ Zhao, CJ Thomas, M Monje Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma Nature, 2017-09-20;549(7673):533-537. 2017-09-20 [PMID: 28959975] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • Q8NFZ3()