Recombinant Human MCEMP1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MCEMP1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of THP‑1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human MCEMP1 protein
  

  Met-Asp	Human IgG1 (Pro100-Lys330)	IEGR	Human MCEMP1 (Val106-Gln187) Accession # NP_777578
  N-terminus			C-terminus

• Accession #: NP_777578
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: MCEMP1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MCEMP1 Fc Chimera Protein, CF

• C19orf59
• chromosome 19 open reading frame 59
• mast cell-expressed membrane protein 1
• MCEMP1
• MGC132456

Background

MCEMP1 (mast cell‑expressed membrane protein-1), or C19orf59 in humans, is a 22 kDa mast cell specific type II transmembrane protein expressed in lung (1). Human MCEMP1 cDNA encodes 187 amino acids (aa) including an 85 aa N‑terminal cytoplasmic region, a 21 aa transmembrane domain, and an 81 aa C‑terminal extracellular domain (ECD, aa 107‑187) that includes one potential N-glycosylation site. One potential 204 aa splice variant contains a 22 aa substitution for the C‑terminal five amino acids. Within the ECD, human MCEMP1 shares only 36‑44% aa sequence identity with presumed mouse, rat, canine, porcine and bovine MCEMP1 orthologs (1). The 5’ flanking sequence of human MCEMP1 contains motifs for NF kappa B/Rel and NFAT, indicating that MCEMP1 expression may be enhanced by stimuli that also enhance cytokine production, such as allergic reactions or pathogens (2). In addition to lung mast cells, protein expression of MCEMP1 has been identified in cord blood-derived cultured mast cells, the mast cell leukemia cell line HMC-1, the acute monocytic leukemia cell line THP-1, and primary CD14⁺ monocytes and mast cells within or adjacent to lung tumors (1). It is not found on peripheral blood lymphocytes, lymphocytic cell lines, or epithelial cells (1).

1. Li, K. et al. (2005) Genomics 86:68.
2. Abraham, S.N. et al. (2010) Nat. Rev. Immunol. 10:440.

Bioinformatics

• Gene Symbol: MCEMP1
• Uniprot:
  • NP_777578()