Recombinant Human IGSF21 His-tag Protein, CF

When Recombinant IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera (Catalog # 6636-NX) binds with an ED50 of 20-120 ng/mL.

2 μg/lane of Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-68 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGSF21 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neurexin 2 alpha Fc Chimera (Catalog # 6636-NX) with an ED₅₀ of 20-120 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IGSF21 protein
  Tyr25-Thr455, with a C-terminal 6-His tag
• Accession #: Q96ID5.1
• N-terminal Sequence: Tyr25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW:

  49 kDa

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-68 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGSF21 His-tag Protein, CF

• FLJ41177
• IGSF21
• immunoglobin superfamily, member 21
• immunoglobulin superfamily member 21
• MGC15730
• RP11-121A23.1

Background

IGSF21 (Immunoglobulin superfamily member 21) is an approximately 60 kDa glycoprotein that is a member of the immunoglobulin superfamily. In mouse, two isoforms have been identified. The long isoform is predominantly expressed in the brain, while the short isoform ~35 kDa is expressed in other organs (1). IGSF21 is expressed at both embryonic and postnatal stages (1). Human IGSF21 is synthesized as a 467 amino acid (aa) protein that includes a 24 aa signal peptide. The mature protein, which contains two Ig-like domains, shares 95% aa sequence identity with mouse and rat IGSF21. IGSF21 selectively promotes inhibitory presynaptic differentiation through interaction with presynaptic neurexin2 alpha that may represent an important mechanism of inhibitory synapse development in the brain (1). Thalamic IGSF21 expression is altered in response to FGF8 signaling (2). Genotypes of IGSF21 are also reported to influence the development of diabetic retinopathy (3).

1. Tanabe, Y. et al. (2017) Nat. Commun. 8:408.
2. Botella-Lopez, A. et al. (2019) Brain Struct. Funct. 224:661.
3. Lin, X. et al. (2016) J. Gene Med. 18:282.

Bioinformatics

• Uniprot:
  • Q96ID5.1()