RecA-1 Antibody (HIS52)

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Immunocytochemistry/ Immunofluorescence: RecA-1 Antibody (HIS52) [NB100-64647] - Used as a pan-endothelial marker to monitor the effect of CT treatment on the density of blood vessels in striatal explants. [PMID: ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr
Clone
HIS52
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

RecA-1 Antibody (HIS52) Summary

Immunogen
Stromal cells from rat lymph node
Specificity
Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Publications
Read Publications using NB100-64647.

Reactivity Notes

Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for RecA-1 Antibody (HIS52)

  • RecA protein
  • RecA1
  • RecA2
  • recaLP
  • Recombinase A

Background

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognizes a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell specific antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RecA-1 Antibody (NB100-64647)(18)


Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-64647 Applications Species
Miya, M et al. Age-related decline in label-retaining tubular cells: implication for reduced regenerative capacity after injury in the aging kidney. Am J Physiol Renal Physiol 302: F694-702. 2012 [PMID: 22169012]
Jantaratnotai, N et al. Comparison of Vascular Perturbations in an Abeta-Injected Animal Model and in AD Brain. Int J Alzheimers Dis 2011: 918280. 2011 [PMID: 21969915]
Morin-Brureau, M et al. Epileptiform activity induces vascular remodeling and zonula occludens 1 downregulation in organotypic hippocampal cultures: role of VEGF signaling pathways. J Neurosci 31: 10677-88. 2011 [PMID: 21775611]
Hamdi, H et al. Epicardial adipose stem cell sheets results in greater post-infarction survival than intramyocardial injections. Cardiovasc Res. 2011 May 12. [PMID: 21565832]
Hawthorne, AL et al. The unusual response of serotonergic neurons after CNS Injury: lack of axonal dieback and enhanced sprouting within the inhibitory environment of the glial scar. J Neurosci 31: 5605-16. 2011 [PMID: 21490201]
Szmydynger-Chodobska, J et al. Multiple sites of vasopressin synthesis in the injured brain. J Cereb Blood Flow Metab 31: 47-51. 2011 [PMID: 20959854]
Schodel, J et al. Factor inhibiting HIF limits the expression of hypoxia-inducible genes in podocytes and distal tubular cells. Kidney Int 78: 836-837. 2010 [PMID: 20720525]
Androutsellis-Theotokis, A et al. Angiogenic factors stimulate growth of adult neural stem cells. PLoS ONE 5: 1-7. 2010 [PMID: 20195471]
Benton, RL et al. Transcriptional activation of endothelial cells by TGFbeta coincides with acute microvascular plasticity following focal spinal cord ischaemia/reperfusion injury. ASN Neuro 26: 181-194. 2009 [PMID: 19663807]
March, S et al. Microenvironmental regulation of the sinusoidal endothelial cell phenotype in vitro. Hepatology 50: 920-8. 2009 [PMID: 19585615]
Show All 18 Publications.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for RecA-1 Antibody (NB100-64647). (Showing 1 - 1 of 1 FAQs).

  1. Did you test this Ab also on human endothelial cells (ECs)? I`m looking for an ab, which recognizes only rat ECs and not human.
    • We have not tested this on human cells so we would not be able to say if it would recognize human or not. I have searched for another RecA-1 antibody for you but currently we only have the one.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64647AF405
Alexa Fluor 488 Labeled NB100-64647AF488
Alexa Fluor 647 Labeled NB100-64647AF647
Alexa Fluor 700 Labeled NB100-64647AF700
DyLight 350 Labeled NB100-64647UV
DyLight 405 Labeled NB100-64647V
DyLight 488 Labeled NB100-64647G
DyLight 680 Labeled NB100-64647FR
DyLight 755 Labeled NB100-64647IR

Additional RecA-1 Products

RecA-1 NB100-64647

Research Areas for RecA-1 Antibody (NB100-64647)

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