RecA-1 Antibody (HIS52) - BSA Free

Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) [NB100-64647] - Used as a pan-endothelial marker to monitor the effect of CT treatment on the density of blood vessels in striatal explants. [PMID: 20195471]

• Reactivity: Rt
• Applications: IHC, ICC/IF
• Clone: HIS52
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

RecA-1 Antibody (HIS52) - BSA Free Summary

• Immunogen: Stromal cells from rat lymph node
• Specificity: Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Reactivity Notes

Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: <0.1% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for RecA-1 Antibody (HIS52) - BSA Free

• RecA protein
• RecA1
• RecA2
• recaLP
• Recombinase A

Background

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognizes a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell specific antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RecA-1 Antibody (NB100-64647)(20)

Publications using NB100-64647

• Yu J, Li C, Ding Q et al. Netrin-1 Ameliorates Blood-Brain Barrier Impairment Secondary to Ischemic Stroke via the Activation of PI3K Pathway Front Neurosci. [PMID: 29311781] (IHC-Fr, Rat)
• Haddad Y1, Couture R1. Localization and Interaction between Kinin B1 Receptor and NADPH Oxidase in the Vascular System of Diabetic Rats Front Physiol. 2017-10-31 [PMID: 29163205] (IHC-Fr, Rat)
• Miya, M et al. Age-related decline in label-retaining tubular cells: implication for reduced regenerative capacity after injury in the aging kidney. Am J Physiol Renal Physiol 302: F694-702. 2012-01-01 [PMID: 22169012]
• Jantaratnotai, N et al. Comparison of Vascular Perturbations in an Abeta-Injected Animal Model and in AD Brain. Int J Alzheimers Dis 2011: 918280. 2011-01-01 [PMID: 21969915]
• Morin-Brureau, M et al. Epileptiform activity induces vascular remodeling and zonula occludens 1 downregulation in organotypic hippocampal cultures: role of VEGF signaling pathways. J Neurosci 31: 10677-88. 2011-01-01 [PMID: 21775611]
• Hamdi, H et al. Epicardial adipose stem cell sheets results in greater post-infarction survival than intramyocardial injections. Cardiovasc Res. 2011-05-12 [PMID: 21565832]
• Hawthorne, AL et al. The unusual response of serotonergic neurons after CNS Injury: lack of axonal dieback and enhanced sprouting within the inhibitory environment of the glial scar. J Neurosci 31: 5605-16. 2011-01-01 [PMID: 21490201]
• Szmydynger-Chodobska, J et al. Multiple sites of vasopressin synthesis in the injured brain. J Cereb Blood Flow Metab 31: 47-51. 2011-01-01 [PMID: 20959854]
• Schodel J, Bohr D, Klanke B et al. Factor inhibiting HIF limits the expression of hypoxia-inducible genes in podocytes and distal tubular cells. Kidney Int. 2010-01-01 [PMID: 20720525]
• Androutsellis-Theotokis, A et al. Angiogenic factors stimulate growth of adult neural stem cells. PLoS ONE 5: 1-7. 2010-01-01 [PMID: 20195471]
• Benton, RL et al. Transcriptional activation of endothelial cells by TGFbeta coincides with acute microvascular plasticity following focal spinal cord ischaemia/reperfusion injury. ASN Neuro 26: 181-194. 2009-01-01 [PMID: 19663807]
• March, S et al. Microenvironmental regulation of the sinusoidal endothelial cell phenotype in vitro. Hepatology 50: 920-8. 2009-01-01 [PMID: 19585615]
• Cattaruzza, F et al. Endothelin-converting enzyme 1 promotes re-sensitization of neurokinin 1 receptor-dependent neurogenic inflammation. Br J Pharmacol 156: 730-9. 2009-01-01 [PMID: 19222484]
• Valable, S et al. MRI assessment of hemodynamic effects of angiopoietin-2 overexpression in a brain tumor model. Neuro Oncol 11: 488-502. 2009-01-01 [PMID: 19168695]
• Sambuceti G, Morbelli S, Vanella L et al. Diabetes Impairs the Vascular Recruitment of Normal Stem Cells by Oxidant Damage, Reversed by Increases in pAMPK, Heme Oxygenase-1, Adiponectin. Stem Cells;27(2):399-407. 2009-01-01 [PMID: 19038792]
• Bexell, D et al. Bone marrow multipotent mesenchymal stroma cells act as pericyte-like migratory vehicles in experimental gliomas. Mol Ther 17: 183-90. 2009-01-01 [PMID: 18985030]
• Alam, SM et al. Decidual cells produce a heparin-binding prolactin family cytokine with putative intrauterine regulatory actions. J Bio Chem 283: 18957-18968. 2008-01-01 [PMID: 18467328]
• Konno, T et al. Pregnancy in the brown Norway rat: a model for investigating the genetics of placentation. Biology of Reproduction 76: 709-718. 2007-01-01 [PMID: 17202390]
• A Hishida et al. Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat. Am. J. Pathol. 158, 75. 2001-01-01 [PMID: 11141481]
• Duijvestein, AM et al. Antibodies defining rat endothelial cells: RECA-1 a pan-endothelial cell specific monoclonal antibody. Lab Invest 66: 459. 1992-01-01 [PMID: 1583886]

FAQs for RecA-1 Antibody (NB100-64647). (Showing 1 - 1 of 1 FAQs).

1. Did you test this Ab also on human endothelial cells (ECs)? I`m looking for an ab, which recognizes only rat ECs and not human.
   • We have not tested this on human cells so we would not be able to say if it would recognize human or not. I have searched for another RecA-1 antibody for you but currently we only have the one.