RBM20 Antibody

Western Blot: RBM20 Antibody [NBP2-27509] - (0.3ug/ml) staining of HEK293 cell lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RBM20 Antibody Summary

• Description: Novus Biologicals Goat RBM20 Antibody (NBP2-27509) is a polyclonal antibody validated for use in WB and ELISA. Anti-RBM20 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QLDKAELDERP corresponding to internal region according to NP_001127835.1.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RBM20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:164000
  • Western Blot 0.3-1 ug/ml
• Application Notes: Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBM20 Antibody

• RNA binding motif protein 20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBM20 Antibody (NBP2-27509)(3)

Publications using NBP2-27509

• Li P, Qin D, Chen T et al. Dysregulated Rbfox2 produces aberrant splicing of CaV1.2 calcium channel in diabetes-induced cardiac hypertrophy Cardiovascular diabetology 2023-07-06 [PMID: 37415128]
• Nishiyama T, Zhang Y, Cui M et al. Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy Science translational medicine 2022-11-23 [PMID: 36417486] (WB, Human)
• Li D, Morales A, Gonzalez-Quintana J et al. Identification of novel mutations in RBM20 in patients withdilated cardiomyopathy. Clin Transl Sci 2010-06-01 [PMID: 20590677]

Bioinformatics

• Gene Symbol: RBM20
• Entrez:
  • 73713(Mouse)
  • 309544(Rat)
• Uniprot:
  • NP_001127835.1(Human)