Pseudorabies Virus Antibody

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Immunohistochemistry: Pseudorabies Virus Antibody [NB120-3534] - Staining of rat hypothalamus from animals infected by injection of PRV into the vitreous body of the eye.

Product Details

Summary
Reactivity Hu, Rt, Ca, V-ViSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Pseudorabies Virus Antibody Summary

Immunogen
Purified pseudorabies virus-Bartha.
Specificity
Pseudorabies Virus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1.0 ug/ml
  • Western Blot 0.2 - 1.0 ug/ml
Application Notes
WB: Detects an approx. 80 - 115 kDa protein representing various PRV proteins. IHC: Staining of PRV in rat hypothalamus from animals injected with PRV into the vitreous body of the eye results in intense staining of neurons in the superchiasmatic nucleus.

Reactivity Notes

Human reactivity reported in scientific literature (see J Virolet all 1988). Rat reactivity reported in scientific literature (see J Neurotrauma.et all 2011).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Pseudorabies Virus Antibody

  • PRV

Background

Herpes simplex virus (HSV), varicella-zoster virus (VZV) and pseudorabies virus (PRV) comprise a family of neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species. Humans and apes are refractory to PRV infection. PRV infects the epithelium of the host where it then spreads to axons of the peripheral nervous system (PNS). The virus opportunistically avails itself of the neuronal retrograde transport pathway which can facilitate transfer to the brain. This unique aspect of PRV allows it to be used as a powerful research tool to study neural connectivity and trace neuronal circuitry in the peripheral (PNS) and central nervous system (CNS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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