Bovine Progesterone ELISA Kit (Colorimetric)

ELISA: Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Bovine Progesterone ELISA Kit (Colorimetric) [NBP2-60122] - Standard Curve Reference

• Reactivity: Bv
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 1 ng/mL - 70 ng/mL
• Sensitivity: 0.2 ng/mL

Bovine Progesterone ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of bovine PROG. No significant cross-reactivity or interference between bovine PROG and analogues was observed.
• Standard Curve Range: 1 ng/mL - 70 ng/mL
• Sensitivity: 0.2 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Bovine Progesterone ELISA Kit (Colorimetric)

• 4-Pregnen-3,20-dione
• Progesterone

Background

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Progesterone ELISA Kit (NBP2-60122)(3)

Publications using NBP2-60122

• Bowolaksono A, Fauzi M, Sundari AM et al. The effects of luteinizing hormone as a suppression factor for apoptosis in bovine luteal cells in vitro Reproduction in domestic animals = Zuchthygiene 2021-02-09 [PMID: 33560544] (ELISA)
• Yi J, Yum S, Kim D et al. Differences in Hormone Levels Around Parturition in Hanwoo (Bos Taurus Coreanae) Following Artificial Insemination and Embryo Transfer Research Square May 4 2021 (ELISA)
• Yi J, Yum SY, Kim D et al. Differences in hormone levels around parturition in Hanwoo cattle (Bos taurus coreanae) following artificial insemination and embryo transfer Veterinary medicine and science 2022-02-22 [PMID: 35193169] (ELISA)