![Western Blot: PPTC7 Antibody [NBP1-90654] - Analysis using Anti-PPTC7 antibody NBP1-90654 (A) shows similar pattern to independent antibody NBP1-90653 (B).](http://images.novusbio.com/fullsize/PPTC7-Antibody-Western-Blot-NBP1-90654-img0010.jpg "Western Blot: PPTC7 Antibody [NBP1-90654] - Analysis using Anti-PPTC7 antibody NBP1-90654 (A) shows similar pattern to independent antibody NBP1-90653 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Knockout (KO) Validated Rabbit PPTC7 Antibody - BSA Free (NBP1-90654) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PPTC7 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: ILTTSYCELLQNKVPLLGSSTACIVVLDRTSHRLHTANLGDSGFLVVRGGEVVHRSDEQQHYFNTPFQLSIAPPEAEGVVLSDSPDAADSTSFDVQL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPTC7 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PPTC7 |
![Immunocytochemistry/Immunofluorescence: PPTC7 Antibody [NBP1-90654] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90654-img0008.jpg "Immunocytochemistry/Immunofluorescence: PPTC7 Antibody [NBP1-90654] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Immunohistochemistry-Paraffin-NBP1-90654-img0013.jpg "Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.")
![Western Blot: PPTC7 Antibody [NBP1-90654] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Western-Blot-NBP1-90654-img0009.jpg "Western Blot: PPTC7 Antibody [NBP1-90654] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Immunohistochemistry-Paraffin-NBP1-90654-img0011.jpg "Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Immunohistochemistry-Paraffin-NBP1-90654-img0012.jpg "Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PPTC7-Antibody-Immunohistochemistry-Paraffin-NBP1-90654-img0014.jpg "Immunohistochemistry-Paraffin: PPTC7 Antibody [NBP1-90654] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")