Polyoma Virus, Medium T Antigen Antibody (PyMT) - BSA Free

Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review.

Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5x magnification in a 6 x 6 tile, scale bar = 2000 um; images enlarged from the boxed regions are at 10x magnification, scale bar = 200 um). Image collected and cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-192), licensed under a CC-BY license.

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - AREG not expressed in PyMT lesions. Representative images of PyMT immunofluorescent staining of 6-week-old AREG +/+ PyMT and AREG -/- PyMT MFPs, respectively. Scale bar shows 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30367629/) licensed under a CC-BY license.

Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. (D) Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5× magnification in a 6 × 6 tile, scale bar = 2000 μm; images enlarged from the boxed regions are at 10× magnification, scale bar = 200 μm). Tumor numbers correspond to the numbering in panel C.

Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - AREG not expressed in PyMT lesions. a Tissue sections hybridized in situ with AREG probe (dots in ducts), & PyMT protein detected by immunofluorescence. Individual channels shown in gray scale; merged image shows AREG in red & PyMT in green. Ductal structures or lesions outlined in white, & labeling of surrounding adipose tissue is nonspecific background staining. Scale bar shows 50 μm. b Representative images of PyMT immunofluorescent staining of 6-week-old AREG+/+ PyMT & AREG−/− PyMT MFPs, respectively. Scale bar shows 100 μm. c At least 10 mammary glands were analyzed for PyMT staining intensity. Statistical analysis performed using t test. AREG amphiregulin, n.s. not significant, PYMT polyoma middle-T antigen, TEB terminal end bud Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30367629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: V-Vi
• Applications: WB, ICC/IF, IHC, IP, Dual ISH-IHC
• Clone: PyMT
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Polyoma Virus, Medium T Antigen Antibody (PyMT) - BSA Free Summary

• Description: Novus Biologicals Rat Polyoma Virus, Medium T Antigen Antibody (PyMT) - BSA Free (NB100-2749) is a monoclonal antibody validated for use in IHC, WB, Dual RNAscope ISH-IHC, ICC/IF and IP. Anti-Polyoma Virus, Medium T Antigen Antibody: Cited in 13 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
• Localization: Cytoplasmic location in cells infected with virus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC reported in scientific literature (PMID 30367629)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 19816927)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Polyoma Virus, Medium T Antigen Antibody (PyMT) - BSA Free

• Middle t antigen
• MPyVgp2
• Polyoma virus middle T antigen
• Polyomavirus medium t antigen
• Polyomavirus middle t antigen

Background

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Polyoma Virus, Medium T Antigen Antibody (NB100-2749)(13)

Publications using NB100-2749

• Passarelli MC, Pinzaru AM, Asgharian H et al. Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics Nature cell biology 2022-03-01 [PMID: 35288656] (WB)
• Borriello L, Coste A, Traub B et al. Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells Nature communications 2022-02-02 [PMID: 35110548] (IF/IHC, Virus)
• Park MK, Zhang L, Min KW et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis Cell metabolism 2021-12-07 [PMID: 34879239] (IF/IHC)
• Williams MM, Christenson JL, O'Neill KI et al. MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer NPJ breast cancer 2021-05-27 [PMID: 34045467] (ICC/IF)
• Tavora B, Mederer T, Wessel KJ et al. Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis Nature 2020-10-01 [PMID: 32999457]
• Bueno MJ, Jimenez-Renard V, Samino S et al. Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells Nat Commun. 2019-11-01 [PMID: 31676791] (WB, Mouse)
• Mao SPH, Park M, Cabrera RM et al. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res. 2018-10-26 [PMID: 30367629] (Dual ISH-IHC, Mouse)
• Joshi S, Yang J, Wang Q et al. 14-3-3zeta loss impedes oncogene-induced mammary tumorigenesis and metastasis by attenuating oncogenic signaling. Am J Cancer Res 2017-09-01 [PMID: 28861322] (WB, Mouse)
  
  Details:
  The Novus anti-PyMT antibody was used for the immunoblot analysis of PyMT levels in PyMT/14-3-3 positive and PyMT/14-3-3 negative mice
• Christenson JL, Butterfield KT, Spoelstra NS et al. MMTV-PyMT and Derived Met-1 Mouse Mammary Tumor Cells as Models for Studying the Role of the Androgen Receptor in Triple-Negative Breast Cancer Progression. Horm Cancer. 2017-02-13 [PMID: 28194662]
• Christenson JL, Kane SE. Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue Mol. Cancer 2014-08-25 [PMID: 25128420]
• Gao D, Joshi N, Choi H et al. Myeloid progenitor cells in the premetastatic lung promote metastases by inducing mesenchymal to epithelial transition. Cancer Research. 2012-01-26 [PMID: 22282653]
• Guest I, Ilic Z, Ma J et al. Direct and indirect contribution of bone marrow-derived cells to cancer. Int J Cancer. 2010-05-15 [PMID: 19816927] (IHC-P)
• Dilworth SM, Griffin BE. Monoclonal antibodies against polyoma virus tumor antigens. Proc Natl Acad Sci USA. 1982-02-01 [PMID: 6175960] (IP)

Review for Polyoma Virus, Medium T Antigen Antibody (NB100-2749) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer ICC Mouse 10/29/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse mammary carcinoma (whole cell lysate)
• Species: Mouse
• Lot: B-01

Comments

• Comments: PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum.