Polyoma Virus, Medium T Antigen Antibody (PyMT)

Images

 
Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good ...read more
Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were ...read more
Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - AREG not expressed in PyMT lesions. Representative images of PyMT immunofluorescent staining of 6-week-old AREG +/+ PyMT and AREG -/- ...read more
Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. (D) Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5× magnification in a 6 × 6 tile, scale bar = 2000 μm; ...read more
Human tumor microarrays (TMAs).Pilocytic astrocytoma TSPO immunohistochemistry (A) and TSPO immunohistochemical scoring (C). Glioblastoma multiforme TSPO immunohistochemistry (B) and TSPO immunohistochemical scoring ...read more

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ICC/IF, IHC, IP, Dual ISH-IHC
Clone
PyMT
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Polyoma Virus, Medium T Antigen Antibody (PyMT) Summary

Immunogen
Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
Localization
Cytoplasmic location in cells infected with virus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC reported in scientific literature (PMID 30367629)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 19816927)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 1 Review rated 4
using
NB100-2749 in the following applications:

Publications
Read Publications using
NB100-2749 in the following applications:

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Polyoma Virus, Medium T Antigen Antibody (PyMT)

  • Middle t antigen
  • MPyVgp2
  • Polyoma virus middle T antigen
  • Polyomavirus medium t antigen
  • Polyomavirus middle t antigen

Background

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Polyoma Virus, Medium T Antigen Antibody (NB100-2749)(13)

We have publications tested in 2 confirmed species: Mouse, Virus.

We have publications tested in 6 applications: Dual ISH-IHC, ICC/IF, IF/IHC, IHC-P, IP, WB.


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Dual ISH-IHC
(1)
ICC/IF
(1)
IF/IHC
(2)
IHC-P
(1)
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(1)
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(3)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-2749 Applications Species
Passarelli MC, Pinzaru AM, Asgharian H et al. Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics Nature cell biology 2022-03-01 [PMID: 35288656] (WB) WB
Borriello L, Coste A, Traub B et al. Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells Nature communications 2022-02-02 [PMID: 35110548] (IF/IHC, Virus) IF/IHC Virus
Park MK, Zhang L, Min KW et al. NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis Cell metabolism 2021-12-07 [PMID: 34879239] (IF/IHC) IF/IHC
Williams MM, Christenson JL, O'Neill KI et al. MicroRNA-200c restoration reveals a cytokine profile to enhance M1 macrophage polarization in breast cancer NPJ breast cancer 2021-05-27 [PMID: 34045467] (ICC/IF) ICC/IF
Tavora B, Mederer T, Wessel KJ et al. Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis Nature 2020-10-01 [PMID: 32999457]
Bueno MJ, Jimenez-Renard V, Samino S et al. Essentiality of fatty acid synthase in the 2D to anchorage-independent growth transition in transforming cells Nat Commun. 2019-11-01 [PMID: 31676791] (WB, Mouse) WB Mouse
Mao SPH, Park M, Cabrera RM et al. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res. 2018-10-26 [PMID: 30367629] (Dual ISH-IHC, Mouse) Dual ISH-IHC Mouse
Joshi S, Yang J, Wang Q et al. 14-3-3zeta loss impedes oncogene-induced mammary tumorigenesis and metastasis by attenuating oncogenic signaling. Am J Cancer Res 2017-09-01 [PMID: 28861322] (WB, Mouse)

Details:
The Novus anti-PyMT antibody was used for the immunoblot analysis of PyMT levels in PyMT/14-3-3 positive and PyMT/14-3-3 negative mice
WB Mouse
Christenson JL, Butterfield KT, Spoelstra NS et al. MMTV-PyMT and Derived Met-1 Mouse Mammary Tumor Cells as Models for Studying the Role of the Androgen Receptor in Triple-Negative Breast Cancer Progression. Horm Cancer. 2017-02-13 [PMID: 28194662]
Christenson JL, Kane SE. Darpp-32 and t-Darpp are differentially expressed in normal and malignant mouse mammary tissue Mol. Cancer 2014-08-25 [PMID: 25128420]
Show All 13 Publications.

Review for Polyoma Virus, Medium T Antigen Antibody (NB100-2749) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-2749:
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ICC
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Polyoma Virus, Medium T Antigen NB100-2749
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4
reviewed by:
Verified Customer
ICC Mouse 10/29/2018
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Summary

ApplicationImmunocytochemistry
Sample TestedMouse mammary carcinoma (whole cell lysate)
SpeciesMouse
LotB-01

Comments

CommentsPyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum.

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Verified Customer
10/29/2018
Application: ICC
Species: Mouse