Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review. Image from the standard format of this antibody.

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5x magnification in a 6 x 6 tile, scale bar = 2000 um; images enlarged from the boxed regions are at

• Reactivity: V-Vi
• Applications: WB, ICC/IF, IHC, IP
• Clone: PyMT
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free Summary

• Immunogen: Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
• Localization: Cytoplasmic location in cells infected with virus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 19816927)
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

• Middle t antigen
• MPyVgp2
• Polyoma virus middle T antigen
• Polyomavirus medium t antigen
• Polyomavirus middle t antigen

Background

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.