Polyoma Virus, Medium T Antigen Antibody (PyMT) [DyLight 405]

• Reactivity: V-Vi
• Applications: WB, ICC/IF, IHC, IP, Dual ISH-IHC
• Clone: PyMT
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 405

Polyoma Virus, Medium T Antigen Antibody (PyMT) [DyLight 405] Summary

• Immunogen: Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
• Localization: Cytoplasmic location in cells infected with virus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Polyoma Virus, Medium T Antigen Antibody (PyMT) [DyLight 405]

• Middle t antigen
• MPyVgp2
• Polyoma virus middle T antigen
• Polyomavirus medium t antigen
• Polyomavirus middle t antigen

Background

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.