PLA2G4B Antibody (1G5)

Images

 
Western Blot: PLA2G4B Antibody (1G5) [H00008681-M01] - Analysis of PLA2G4B expression in transfected 293T cell line by PLA2G4B monoclonal antibody (M01), clone 1G5.Lane 1: PLA2G4B transfected lysate(114.121 KDa).Lane 2: ...read more
Sandwich ELISA: PLA2G4B Antibody (1G5) [H00008681-M01] - Detection limit for recombinant GST tagged PLA2G4B is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PLA2G4B Antibody (1G5) Summary

Immunogen
PLA2G4B (AAH25290.1, 1 a.a. - 316 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEAALEAVRSELREFPAAARELCVPLAVPYLDKPPTPLHFYRDWVCPNRPCIIRNALQHWPALQKWSLPYFRATVGSTEVSVAVTPDGYADAVRGDRFMMPAERRLPLSFVLDVLEGRAQHPGVLYVQKQCSNLPSELPQLLPDLESHVPWASEALGKMPDAVNFWLGEAAAVTSLHKDHYENLYCVVSGEKHFLFHPPSDRPFIPYELYTPATYQLTEEGTFKVVDEEAMEKVPWIPLDPLAPDLARYPSYSQAQALRCTVRAGEMLYLPALWFHHVQQSQGCIAVNFWYDMEYDLKYSYFQLLDSLTKASGLD
Specificity
PLA2G4B (1G5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
JMJD7-PLA2G4B
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00008681-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PLA2G4B Antibody (1G5)

  • FLJ20543
  • FLJ20656
  • FLJ20807
  • FLJ77014
  • FLJ78330
  • jmjC domain-containing protein 7
  • jumonji domain containing 7
  • Jumonji domain-containing protein 7

Background

The LOC100137047-PLA2G4B mRNAs are naturally occurring co-transcribed products of the neighboring LOC100137047 and PLA2G4B genes. Incompletely processed read-through transcripts from these two loci are abundantly expressed in most tissues, but the function of the predicted protein product has not yet been determined. Alternative splicing of this gene results in different transcript variants, some of which are candidates for nonsense-mediated decay (NMD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00008398-D01P
Species: Hu, Mu
Applications: WB
NBP2-32569
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85480
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-83966
Species: Hu
Applications: WB, IHC, IHC-P
AF4925
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-19809
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for PLA2G4B Antibody (H00008681-M01) (0)

There are no publications for PLA2G4B Antibody (H00008681-M01).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for PLA2G4B Antibody (H00008681-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00008681-M01:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PLA2G4B H00008681-M01
Enlarge
reviewed by:
Anonymous
WB Human 01/11/2012
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-mouse IgG-HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL prime

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PLA2G4B Antibody (H00008681-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for PLA2G4B Antibody (H00008681-M01)

Discover related pathways, diseases and genes to PLA2G4B Antibody (H00008681-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Research Areas for PLA2G4B Antibody (H00008681-M01)

Find related products by research area.

Blogs on PLA2G4B

There are no specific blogs for PLA2G4B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/11/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol JMJD7-PLA2G4B
Entrez
OMIM
Uniprot