Phosphoenolpyruvate Carboxylase Antibody

Western Blot: Phospho Enol Pyruvate Carboxylase Antibody [NBP2-44235] - lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane.

• Reactivity: Pl
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Phosphoenolpyruvate Carboxylase Antibody Summary

• Immunogen: Phosphoenolpyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase. (Uniprot: B8XPZ2)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOC542372
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:40000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000-1:4000
• Application Notes: This product has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Phosphoenolpyruvate Carboxylase Antibody

• PEPC 1
• PEPC
• PEPC1
• PEPCase 1
• PEPCase
• Phosphoenolpyruvate carboxylase 1
• PPC1
• PPEPC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LOC542372
• Uniprot:
  • B8XPZ2(Plant)