Phosphoenolpyruvate Carboxylase Antibody

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Western Blot: Phospho Enol Pyruvate Carboxylase Antibody [NBP2-44235] - lane 1: Phospho Enol Pyruvate (PEP) Carboxylase. Lane 2: None. Load: 50 ng per lane.

Product Details

Summary
Reactivity PlSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Phosphoenolpyruvate Carboxylase Antibody Summary

Immunogen
Phosphoenolpyruvate Carboxylase Antibody was produced by repeated immunizations with maize leaves Phospho-enol-pyruvate Carboxylase. (Uniprot: B8XPZ2)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LOC542372
Purity
Multi-step
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Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:40000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000-1:4000
Application Notes
This product has been tested by western blotting and ELISA is suitable for IHC. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Phosphoenolpyruvate Carboxylase Antibody

  • PEPC 1
  • PEPC
  • PEPC1
  • PEPCase 1
  • PEPCase
  • Phosphoenolpyruvate carboxylase 1
  • PPC1
  • PPEPC4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LOC542372
Uniprot