![Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunoprecipitation of EGF-treated A431 cells by Clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111.](http://images.novusbio.com/fullsize/nbp3-25981_rabbit-phospho-tyrosine-mab-rm111-azide-and-bsa-free-2262024231105.jpg "Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunoprecipitation of EGF-treated A431 cells by Clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111.")
| Reactivity | AllSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP, ChIP |
| Clone | RM111 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Phospho-Tyrosine -BSA conjugate |
| Specificity | This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Western blot of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.](http://images.novusbio.com/fullsize/nbp3-25981_rabbit-phospho-tyrosine-mab-rm111-azide-and-bsa-free-22620242232127.jpg "Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Western blot of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.")
![Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-25981_rabbit-phospho-tyrosine-mab-rm111-azide-and-bsa-free-22620242311082.jpg "Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).")
![Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 reacts only to phosphorylated Tyrosine.](http://images.novusbio.com/fullsize/nbp3-25981_rabbit-phospho-tyrosine-mab-rm111-azide-and-bsa-free-24620241332449.jpg "Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 reacts only to phosphorylated Tyrosine.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")