Phospho-Tyrosine Antibody (P-TYR-01) [DyLight 650]

• Reactivity: All-NA
• Applications: WB, Flow, ICC/IF
• Clone: P-TYR-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 650

Phospho-Tyrosine Antibody (P-TYR-01) [DyLight 650] Summary

• Immunogen: Phosphotyrosine conjugated to BSA.
• Specificity: The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Various species

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Phospho-Tyrosine Antibody (P-TYR-01) [DyLight 650]

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.