Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (P-TYR-01) [NB500-335] - Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody-Alexa Fluor 488 (green). Actin detected by phalloidin-Alexa Fluor 567, DNA stained by DAPI (blue).

• Reactivity: All-NA
• Applications: WB, Flow, ICC/IF
• Clone: P-TYR-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free Summary

• Description: Novus Biologicals Mouse Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free (NB500-335) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Phosphotyrosine conjugated to BSA.
• Specificity: The antibody P-Tyr-01 detects tyrosine phosphorylation in activated cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 2-5 ug/ml
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1-2 ug/ml

Reactivity Notes

Various species

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Phospho-Tyrosine Antibody (P-TYR-01) - BSA Free

• 2 amino 3(4 hydroxyphenyl) propanoic acid
• 4 hydroxyphenylalanine
• PhosphoTyrosine
• pTyrosine
• pY20
• Tyrosine

Background

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.