Phantom Antibody

• Reactivity: Dr
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.75 mg/ml

Phantom Antibody Summary

• Description: Novus Biologicals Rabbit Phantom Antibody (NB300-658) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide for Saxophone antibodies are (WIT-101AP XXXXXXXXXXXXXXXXXX and; WIT-112APXXXXXXXXXXXXXXXXXXXXXXX; All peptides were amidated for conjugation.
• Specificity: Phantom
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Immunocytochemistry, Immunoprecipitation and Western Blot: Optimal dilutions and concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Drosophilia melanogaster.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.75 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Phantom Antibody

• Exo
• Exocephalon

Background

Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog & wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promote its cell division in the female germline (1, 2). The Signaling by TGF beta-related factors requires ligand-induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the saxophone (sax) and thick veins (tkv) genes encode type I receptors that mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. Where as Punt and Wishful Thinking (wit) are type II receptors. Over expression or mutation in dpp suppress germline stem cell differentiation. Dpp actions are mediated by its receptor Saxophone. The Saxophone gene is expressed ubiquitously. The Saxophone gene also gives two products Brk43E and Berk25 and both gene products inter acts with TGF super family peptide ligands Dpp. Mutations that completely abolish Saxophone activity causes phenotype that are similar to partial or complete loss of activity of the dpp ligand. The saxophone products are also have serine/threonine kinase activity responsible for phsosphorylation and activation of the ligands including pMAD, Screw (Scw), and short gastrulation protein (Sog). The mutant larvae showed small synapses, defective evoke potentials and slower vesicular release and changes in the ultra-structural architect (3). Mutations in the Phantom gene disrupts the neuronal remodeling in mushroom bodies (MBs) in fruit fly (4). The Anti-Phantom-selective antibodies were generated against conserved sequences near the N-terminus and the C-terminal end of the protein that are unique to D. melanogaster Phantom protein. The Anti-Phantom-selective antibodies are affinity purified against immobilized antigen based affinity chromatography which yielded epitope-specific antibodies. Anti-Phantom-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.