![Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0005.jpg "Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.")
| Reactivity | PoSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-614-0.1mg | |||
| HRP | NB120-20732-0.1mg | |||
| Description | This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach] Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Multi-step |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Storage | Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Multi-step |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent) |
Secondary Antibodies |
Isotype Controls |
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| Uniprot |
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![Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).](http://images.novusbio.com/fullsize/Pepsin-Antibody-Immunohistochemistry-Paraffin-NBP2-44230-img0001.jpg "Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).")
![Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0002.jpg "Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.")
![Western Blot: Pepsin Antibody [NBP2-44230] - Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0003.jpg "Western Blot: Pepsin Antibody [NBP2-44230] - Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin. Image using the Biotin form of this antibody.")
