PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

Western Blot: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa PCDHGA1 protein using Mouse Anti-PCDHGA1 Monoclonal Antibody, Clone S159-5 (NBP2-42207). Load: 10 ug. Primary Antibody: Mouse Anti-PCDHGA1 Monoclonal Antibody (NBP2-42207) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.

Immunocytochemistry/Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody, Clone S159-5 (NBP2-42207). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody (NBP2-42207) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PCDHGA1 (pan) Antibody (D) Composite.

Western Blot: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - analysis of Rat Brain Membrane showing detection of 100 kDa Protocadherin Gamma protein using Mouse Anti-Protocadherin Gamma Monoclonal Antibody, Clone S159-5 . Load: 10 ug. Primary Antibody: Mouse Anti-Protocadherin Gamma Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: 100 kDa.

Immunocytochemistry/Immunofluorescence: PCDHGA1 Antibody (S159-5) - Pan Specific [NBP2-42207] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody, Clone S159-5 (NBP2-42207). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PCDHGA1 (pan) Monoclonal Antibody (NBP2-42207) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Nucleus . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) PCDHGA1 (pan) Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S159-5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free Summary

• Description: Novus Biologicals Mouse PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free (NBP2-42207) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
• Localization: Cell Membrane
• Specificity: Detects approx 100kDa. Cross-reacts with all Gamma-protocadherin-A, -B and -C proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PCDHGA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Protocadherin Gamma (pan) Antibody was sufficient for detection of Protocadherin gamma (pan) in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PCDHGA1 Antibody (S159-5) - Pan Specific - BSA Free

• MGC138287
• MGC138289
• PCDH-GAMMA-A1
• protocadherin gamma subfamily A, 1
• protocadherin gamma-A1

Background

The PCDHGA1 gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked onchromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism maybe involved in their regulat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PCDHGA1