Pax3/Pax7 Antibody [Unconjugated]

Pax3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Pax3/Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2457) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus of melanocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Pax3/Pax7 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Pax3
  Met1-Ser215
  Accession # NP_000429
• Specificity: Detects Pax3/Pax7 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5, under reducing conditions, is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PAX3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pax3/Pax7 Antibody [Unconjugated]

• Pax3/Pax7

Background

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. There is 98% homology between human and mouse Pax3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax3/Pax7 Antibody (AF2457)(5)

Publications using AF2457

• S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion Oncogene, 2022-07-19;40(3):111119. 2022-07-19 [PMID: 35858555] (IHC, Human)
• Sandeep Gupta, Ken Yamauchi, Bennett G. Novitch, Samantha J. Butler Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells STAR Protocols 2021-03-04 [PMID: 33659900]
• John J. Young, Phil Grayson, Scott V. Edwards, Clifford J. Tabin Attenuated Fgf Signaling Underlies the Forelimb Heterochrony in the Emu Dromaius novaehollandiae Current Biology 2019-11-01 [PMID: 31668620]
• Hazen VM, Phan KD, Hudiburgh S, Butler SJ Inhibitory Smads differentially regulate cell fate specification and axon dynamics in the dorsal spinal cord. Dev. Biol., 2011-06-22;356(2):566-75. 2011-06-22 [PMID: 21718693] (IHC, Chicken)
• Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin. Stem Cells, 2007-09-27;26(1):163-72. 2007-09-27 [PMID: 17901404] (ICC, Human)

Bioinformatics

• Gene Symbol: PAX3
• Uniprot:
  • NP_000429()