Pax3/Pax7 Antibodies: Novus Biologicals

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3 Results for "pax3-pax7" in Primary Antibodies

MAB2457

Pax3/Pax7 Antibody (274212) [Unconjugated]

(1 Review)

(19 Publications)

100 μg

MAB2457

B16-F1 cells were stained with Mouse Anti-Human/Mouse Pax3/Pax7 /Pax7 Monoclonal Antibody (Catalog # MAB2457, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Pax3/Pax7 was detected in immersion fixed B16-F1 mouse melanoma cell line using Mouse Anti-Human/Mouse Pax3/Pax7 Monoclonal Antibody (Catalog # MAB2457) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Characterization of iMPCs during monolayer differentiation. a–e Representative immunostaining of Pax3 (a), Myf5 (b), MyoD (c), and MyoG (d), and corresponding quantification (e) during iMPC expansion. Scale bar=100 µm. f Representative FACS analysis for CD56 in H9 and TRiPSC derived iMPCs. g Representative immunostaining (top) and quantification (bottom) of Pax7+ and MyoG+ cell populations for H9 and TRiPS derived myotubes at 2 weeks of monolayer differentiation. (n = 6 samples from 2 differentiations for each cell line). h Representative immunostaining and quantification of GFP+/Pax7+ and GFP-/Pax7+ cell pools at 2 weeks of monolayer differentiation. Scale bar=50 µm. (n = 4 samples from 2 differentiations for each cell line). i Representative immunostaining and quantification of myotube diameter at 1, 2, and 4 weeks of monolayer differentiation. (*P < 0.05 vs. 1 week, #P < 0.05 vs. 4 week, Tukey–Kramer HSD test; n = 6 samples from 2 differentiations for each cell line). Scale bars=50 µm. Data are presented as mean ± SEM Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29317646), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent analysis of Pax3 expression in the developing mouse cerebellum.(A) Bar plot showing the percentage of Pax3 + cells co-stained (y-axis) with cerebellar cell markers Pax2, Foxp2 and Calbindin at E12, E15, and P3 (x-axis). (B) Top: Immunofluorescent co-staining of Pax3 (red) and Foxp2 (green) in embryonic cerebellum at E15. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Bottom: Immunofluorescent co-staining of Pax3 (red) and Calb (green) in the postnatal cerebellum at P0. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Labels: VZ: Ventricular zone, EGL: External granular layer, PCL: Purkinje cell layer, ML: Molecular layer, Scalebars = 100 µm. (C) Immunofluorescent staining of Pax3 (red) in the developing cerebellum at P9. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35942939), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent analysis of Pax3 expression in the developing mouse cerebellum.(A) Bar plot showing the percentage of Pax3 + cells co-stained (y-axis) with cerebellar cell markers Pax2, Foxp2 and Calbindin at E12, E15, and P3 (x-axis). (B) Top: Immunofluorescent co-staining of Pax3 (red) and Foxp2 (green) in embryonic cerebellum at E15. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Bottom: Immunofluorescent co-staining of Pax3 (red) and Calb (green) in the postnatal cerebellum at P0. Merged image is a composite image of the Pax3, Foxp2, and DAPI. Labels: VZ: Ventricular zone, EGL: External granular layer, PCL: Purkinje cell layer, ML: Molecular layer, Scalebars = 100 µm. (C) Immunofluorescent staining of Pax3 (red) in the developing cerebellum at P9. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35942939), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2457U-100UG
Alexa Fluor 405	IC2457V-100UG
Alexa Fluor 488	IC2457G-100UG
Alexa Fluor 532	FAB2457X
Alexa Fluor 594	IC2457T-100UG
Alexa Fluor 647	IC2457R-100UG
Alexa Fluor 700	IC2457N-100UG
Alexa Fluor 750	IC2457S-100UG
Allophycocyanin	IC2457A
Biotin	FAB2457B
CoraFluor 1	FAB2457CL1
DyLight 350	FAB2457D
DyLight 405	FAB2457E
DyLight 488	FAB2457K
DyLight 550	FAB2457L
DyLight 594	FAB2457M
DyLight 650	FAB2457W
DyLight 680	FAB2457Y
DyLight 755	FAB2457Z
FITC	FAB2457F
HRP	FAB2457H
Janelia Fluor 525	FAB2457JF525
Janelia Fluor 549	FAB2457I
Janelia Fluor 646	FAB2457J
Janelia Fluor 669	FAB2457JF669
PerCP	FAB2457C
Phycoerythrin	IC2457P
mFluor Violet 450 SE	FAB2457MFV450
mFluor Violet 500 SE	FAB2457MFV500
mFluor Violet 610 SE	FAB2457MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC2457P
Carrier Free	IC2457A
Carrier Free	MAB2457-SP
Carrier Free	IC2457V-100UG
Carrier Free	IC2457U-100UG
Carrier Free	IC2457T-100UG
Carrier Free	IC2457S-100UG
Carrier Free	IC2457R-100UG
Carrier Free	IC2457N-100UG
Carrier Free	IC2457G-100UG

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AF2457

Pax3/Pax7 Antibody [Unconjugated]

(5 Publications)

100 μg

AF2457

Pax3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Pax3/Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2457) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus of melanocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2457U-100UG
Alexa Fluor 405	AF2457V-100UG
Alexa Fluor 488	AF2457G-100UG
Alexa Fluor 532	AF2457X-100UG
Alexa Fluor 594	AF2457T-100UG
Alexa Fluor 647	AF2457R-100UG
Alexa Fluor 700	AF2457N-100UG
Alexa Fluor 750	AF2457S-100UG
Allophycocyanin	AF2457A
Biotin	BAF2457
CoraFluor 1	AF2457CL1
DyLight 350	AF2457D
DyLight 405	AF2457E
DyLight 488	AF2457K
DyLight 550	AF2457L
DyLight 594	AF2457M
DyLight 650	AF2457W
DyLight 680	AF2457Y
DyLight 755	AF2457Z
FITC	AF2457F
HRP	AF2457H
Janelia Fluor 525	AF2457JF525
Janelia Fluor 549	AF2457I
Janelia Fluor 646	AF2457J
Janelia Fluor 669	AF2457JF669
PE	AF2457P
PerCP	AF2457C
mFluor Violet 450 SE	AF2457MFV450
mFluor Violet 500 SE	AF2457MFV500
mFluor Violet 610 SE	AF2457MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2457-SP

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IC2457G

Pax3/Pax7 Antibody (274212) [Alexa Fluor® 488]

100 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor 488

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2457U-100UG
Alexa Fluor 405	IC2457V-100UG
Alexa Fluor 532	FAB2457X
Alexa Fluor 594	IC2457T-100UG
Alexa Fluor 647	IC2457R-100UG
Alexa Fluor 700	IC2457N-100UG
Alexa Fluor 750	IC2457S-100UG
Allophycocyanin	IC2457A
Biotin	FAB2457B
CoraFluor 1	FAB2457CL1
DyLight 350	FAB2457D
DyLight 405	FAB2457E
DyLight 488	FAB2457K
DyLight 550	FAB2457L
DyLight 594	FAB2457M
DyLight 650	FAB2457W
DyLight 680	FAB2457Y
DyLight 755	FAB2457Z
FITC	FAB2457F
HRP	FAB2457H
Janelia Fluor 525	FAB2457JF525
Janelia Fluor 549	FAB2457I
Janelia Fluor 585	FAB2457JF585
Janelia Fluor 635	FAB2457JF635
Janelia Fluor 646	FAB2457J
Janelia Fluor 669	FAB2457JF669
PerCP	FAB2457C
Phycoerythrin	IC2457P
mFluor Violet 450 SE	FAB2457MFV450
mFluor Violet 500 SE	FAB2457MFV500
mFluor Violet 610 SE	FAB2457MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC2457P
Carrier Free	MAB2457
Carrier Free	IC2457A
Carrier Free	MAB2457-SP
Carrier Free	IC2457V-100UG
Carrier Free	IC2457U-100UG
Carrier Free	IC2457T-100UG
Carrier Free	IC2457S-100UG
Carrier Free	IC2457R-100UG
Carrier Free	IC2457N-100UG

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