Parvovirus VP2 Antibody (4E5A4) - BSA Free

Western Blot: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis using VP2 mAb against HEK293 (1) and VP2 (AA: 296-438)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis of Hela cells using VP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis of bladder cancer tissues using VP2 mouse mAb with DAB staining.

Flow Cytometry: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis of Hela cells using VP2 mouse mAb (green) and negative control (red).

Western Blot: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis using VP2 mAb against human VP2 (AA: 296-438) recombinant protein. (Expected MW is 42.1 kDa)

Western Blot: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Analysis using VP2 mouse mAb against A431 (1) and BCBL-1 (2) cell lysate.

ELISA: Parvovirus VP2 Antibody (4E5A4) [NBP2-61902] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: V-Vi
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 4E5A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Parvovirus VP2 Antibody (4E5A4) - BSA Free Summary

• Description: Novus Biologicals Mouse Parvovirus VP2 Antibody (4E5A4) - BSA Free (NBP2-61902) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Parvovirus VP2 (AA: 296-438) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Parvovirus VP2 Antibody (4E5A4) - BSA Free

• Parvovirus capsid protein 2
• Parvovirus major capsid protein

Background

Human parvovirus B19 (B19) is an erythrovirus responsible for acute and chronic anemia in susceptible patients. The virus replicates, both in vivo and in vitro, in the nucleus of the erythroid progenitors. The viral capsid is composed of two viral proteins, VP1 and VP2, the latter making up almost 96% of the viral capsid proteins ;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Parvovirus VP2 Antibody (NBP2-61902) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: canine distemper H protein.

reviewed by: Junjie Shao WB canine distemper H protein 10/20/2020

Summary

• Application: Western Blot
• Sample Tested: lysates from transfected 293T cells
• Species: canine distemper H protein
• Lot: 160630

Comments

• Comments: antibody diluted in 5% milk (in TBS) at 1:5,000.

Bioinformatics

• Gene Symbol: VP2