Pain Antibody

• Reactivity: Dr
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Pain Antibody Summary

• Immunogen: A synthetic peptide from 3rd extracellular loop of drosophila Pain conjugated to an immunogenic carrier protein was used as the immunogen.
• Specificity: Pain (Transient receptor potential cation channel protein painless).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Frozen 1:300-1:2000
  • Immunohistochemistry-Paraffin 1:300-1:2000
  • Western Blot 1:300-1:2000
• Application Notes: Useful in Immunofluorescence (PMID: 22073144).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Pain Antibody

• Painless protein
• Transient protein painless
• Transient receptor potential cation channel protein painless

Background

FUNCTION: Receptor-activated non-selective cation channel involved in detection of pain sensation due to high temperature. Involved in heat nociception by being activated by noxious temperature of 38 degrees Celsius. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Present in multidendritic neurons, chordotonal neurons, a subset of cells in the central nervous system and a subset of sensory neurons in the antennal-maxillary complex. Not detected in gonads and dorsal vessels (at protein level). Expressed in peripheral neurons that extend multiple branched dendrites beneath the larval epidermis, similar to vertebrate pain receptors. DEVELOPMENTAL STAGE: Expressed from stage 13 of embryonic development in a small number of cells in the central nervous system and in a subset of neurons of the peripheral nervous system. Such cells may include sensory precursors of multidendritic (md) neurons. At embryonic stage 16, prior to when dendritic process are elaborated, it appears to be expressed in multidendritic neurons in a polarized manner. At stage 17 of development, when the md neurons first initiate dendritogenesis, it is localized to branched projections initiating from clusters of multidendritic neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pain Antibody (NB100-98737)(1)

Publication using NB100-98737

• Wang K, Guo Y, Wang F, Wang Z. Drosophila TRPA channel painless inhibits male-male courtship behavior through modulating olfactory sensation. PLoS One;6(11). 2011-01-01 [PMID: 22073144]